Tavella Alexander, Zambotto Paolo, Stifter Ernst, Lombardo Dorotea, Rabini Michela, Robatscher Eva, Brem Gottfried
Institut für Tierseuchenbekämpfung der Venetien, Italien.
Berl Munch Tierarztl Wochenschr. 2012 Jul-Aug;125(7-8):326-31.
In 2005 the Autonomous Province of Bolzano implemented a BVD-Virus control programme by testing all newborn calves for Bovine Viral Diarrhoea Virus (BVDV) antigen by analysing ear notch samples. In eradication programs between November 2005 and October 2010 a total of 344,108 newborn calves were ear notch sampled and analysed for BVDV by an antigen ELISA detecting the E(rns) (HerdCheck BVDV Antigen/Serum Plus, Idexx Laboratories, Switzerland). The tissue samples were collected during calf ear tagging (within the first 20 days of life) using two sampling devices (TypiFix-System, Agrobiogen GmbH [Germany] and Allflex [France]). 0.4% (1400) of the collected samples showed a positive result, and of these, 583 calves were subjected to a follow-up examination, analysing the samples again by ELISA as well blood samples by Reverse transcriptase-PCR. Blood samples were also collected from the dam, if still present on the farm by the time of sampling. These results suggest that a BVDV control programme testing all newborn calves sampled for BVDV antigen by analysing an ear notch sample in principle is practicable. However, due to the low positive predictive value (75%) of the E(rns)-ELISA used, not all persistently infected calves can be detected.
2005年，博尔扎诺自治省实施了一项牛病毒性腹泻病毒（BVDV）控制计划，通过分析耳缺刻样本对所有新生犊牛进行牛病毒性腹泻病毒（BVDV）抗原检测。在2005年11月至2010年10月的根除计划中，总共对344,108头新生犊牛进行了耳缺刻采样，并通过检测E(rns)的抗原酶联免疫吸附测定法（HerdCheck BVDV抗原/血清增强版，爱德士生物科技公司，瑞士）对BVDV进行分析。组织样本是在犊牛耳标佩戴期间（出生后20天内）使用两种采样设备（TypiFix系统，德国Agrobiogen有限公司和法国Allflex公司）采集的。0.4%（1400份）的采集样本呈阳性结果，其中583头犊牛接受了后续检查，再次通过酶联免疫吸附测定法分析样本，并通过逆转录聚合酶链反应分析血样。如果在采样时母牛仍在农场，也会采集其血样。这些结果表明，通过分析耳缺刻样本对所有新生犊牛进行BVDV抗原检测的BVDV控制计划原则上是可行的。然而，由于所使用的E(rns) -酶联免疫吸附测定法的阳性预测值较低（75%），并非所有持续感染的犊牛都能被检测到。
