一种中药方剂“甘露饮”通过抑制 PI3K/AKT 和 ERK 信号通路抑制基质金属蛋白酶-2/9 来抑制血管平滑肌细胞迁移。
A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways.
机构信息
Department of Life Sciences, and Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan.
出版信息
BMC Complement Altern Med. 2012 Aug 24;12:137. doi: 10.1186/1472-6882-12-137.
BACKGROUND
This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.
METHODS
VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.
RESULTS
Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.
CONCLUSIONS
These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.
背景
本研究旨在探讨肝络欣丸（GLY）对胎牛血清诱导的血管平滑肌细胞（VSMCs）迁移的影响及其在大鼠颈动脉球囊损伤模型中对新生内膜形成的作用。
方法
用不同浓度的 GLY 处理 VSMCs，然后用流式细胞术分析、明胶酶谱分析、Transwell 分析和 Western blot 分析。对接受球囊损伤的 SD 大鼠进行 H&E 染色分析。
结果
我们的结果表明，GLY 可显著降低新生内膜的厚度。GLY 通过负向调节磷酸化 ERK1/2、PI3K/AKT 和 FAK，抑制非细胞毒性剂量的 VSMCs 迁移。数据表明，GLY 可抑制 VSMCs 细胞迁移，并可能通过抑制 VSMCs 迁移来阻止损伤诱导的新生内膜过度增生，而不诱导细胞凋亡。
结论
这些观察结果为 GLY 减轻细胞迁移的机制提供了依据，因此可能成为再狭窄的潜在干预手段。
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