School of Pharmacy, China Medical University,Taichung 40402, Taiwan.
J Agric Food Chem. 2010 Jul 14;58(13):7685-92. doi: 10.1021/jf1002254.
According to the known effects of each ingredient, Gan-Lu-Yin (GLY), a traditional Chinese herbal formula, has the potential to be an antiangiogenic agent. The purpose of this study was to explore the putative effect of GLY on antiangiogenesis. An ethanol extract of GLY was tested on chicken chorioallantoic membrane (CAM) and human umbilical vein endothelial cells (HUVEC) to evaluate the effects of GLY extract on cell proliferation, migration, and tube formation. The results showed that treatment with 1.0 mg/mL of GLY extract could markedly reduce cell migration and in vitro tube formation of HUVEC, and 1.5 mg/mL of GLY extract was sufficient to inhibit proliferation of HUVEC. The expression level of vascular endothelial growth factor (VEGF) of HUVEC was significantly decreased by 1.5 and 2.0 mg/mL of GLY extract. In chicken CAM assay, all tested concentrations of GLY extract were found to reduce the capillary mesh on the CAM of fertilized eggs. The inhibitory effects of GLY extract (1 mg/mL) were also found on tumor cell-induced HUVEC proliferation and tube formation. These observations suggested that GLY extract has an inhibitory effect on angiogenesis, which in turn may prevent tumor growth, and its mechanism might be partially associated with blocking VEGF protein expression of HUVEC.
根据各成分的已知作用，甘露饮（GLY）作为一种传统的中草药配方，具有成为抗血管生成剂的潜力。本研究旨在探讨 GLY 对血管生成抑制的潜在作用。用 GLY 的乙醇提取物检测鸡胚尿囊膜（CAM）和人脐静脉内皮细胞（HUVEC），以评估 GLY 提取物对细胞增殖、迁移和管状形成的影响。结果表明，1.0 mg/mL 的 GLY 提取物可显著减少 HUVEC 的细胞迁移和体外管状形成，而 1.5 mg/mL 的 GLY 提取物足以抑制 HUVEC 的增殖。1.5 和 2.0 mg/mL 的 GLY 提取物使 HUVEC 的血管内皮生长因子（VEGF）表达水平显著降低。在鸡 CAM 试验中，所有测试浓度的 GLY 提取物均被发现减少了受精卵 CAM 上的毛细血管网。GLY 提取物（1 mg/mL）对肿瘤细胞诱导的 HUVEC 增殖和管状形成也具有抑制作用。这些观察结果表明，GLY 提取物对血管生成具有抑制作用，从而可能防止肿瘤生长，其机制可能部分与阻断 HUVEC 的 VEGF 蛋白表达有关。
