NeuroCritical Care Unit, Virgen del Rocío University Hospital, IBIS/CSIC/University of Seville, Seville, Spain.
J Neurosci Methods. 2012 Oct 15;211(1):88-93. doi: 10.1016/j.jneumeth.2012.08.010. Epub 2012 Aug 19.
Describe the results obtained with a new percutaneous, intracisternal model of Subarachnoid Haemorrhage (SAH) in Wistar rats by a single injection of non-heparinised, autologous blood.
Once anaesthetized the rat was fixed prone in a stereotaxic frame. After identifying the projection of the occipital bone, the needle of the stereotaxic frame aspirated towards the foramen magnum until it punctured through the atlanto-occipital membrane and obtained cerebrospinal fluid. Autologous blood (100 μl) was withdrawn from the tail and injected intracisternally. This procedure was repeated in the sham group, injecting 100 μl of isotonic saline. On the fifth day post-intervention, the rats were anaesthetized and the brain was exposed. After a lethal injection of ketamine the brain was explanted and fixed in paraformaldehyde. Gross and microscopic inspection of the slices revealed the existence or non-existence of pathological findings.
A total of 26 rats were operated on (13 in the SAH group/13 in the sham group). The average time between obtaining the blood and the start of the intracisternal injection was 10 (±1.2)s. The mortality rate was 16.12%. Intra- and extraparenchymal ischemic-haemorrhagic lesions were found in three animals (23.07%)--all from the SAH group--with ischemic neuronal cell injury detected in two of the three.
The new murine model of SAH is easy to perform, with low mortality, minimally invasive, which makes it interesting for future studies on vasospasm-related delayed SAH complications.
描述通过向 Wistar 大鼠单次注射非肝素化自体血建立蛛网膜下腔出血（SAH）的新皮内、蛛网膜下腔模型的结果。
麻醉大鼠后，将其固定在立体定向框架中呈俯卧位。在识别枕骨的投影后，立体定向框架的针头向枕骨大孔方向抽吸，直到穿透寰枕膜并获得脑脊液。从尾巴中抽出 100μl 自体血，并注入蛛网膜下腔。在假手术组中，重复此过程，注入 100μl 等渗盐水。在干预后的第 5 天，对大鼠进行麻醉并暴露大脑。在给予氯胺酮致死剂量后，将大脑取出并固定在多聚甲醛中。对切片进行大体和显微镜检查，以显示是否存在病理学发现。
共对 26 只大鼠进行了手术（SAH 组 13 只/假手术组 13 只）。从获得血液到开始蛛网膜下腔注射的平均时间为 10（±1.2）s。死亡率为 16.12%。在 3 只动物（23.07%）中发现了脑实质内和脑实质外的缺血性出血性病变--均来自 SAH 组--其中 3 只中的 2 只检测到缺血性神经元细胞损伤。
新的 SAH 鼠模型易于操作，死亡率低，微创，这使其成为未来研究与血管痉挛相关的迟发性 SAH 并发症的有趣模型。
