Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong, China.
Diagn Microbiol Infect Dis. 2012 Oct;74(2):190-7. doi: 10.1016/j.diagmicrobio.2012.06.014. Epub 2012 Aug 24.
Phaeoacremonium parasiticum is an environmental dematiaceous mold rarely associated with human infections. We present here 2 cases of P. parasiticum invasive infections, including the first report of P. parasiticum respiratory tract infection, and 1 case of airway colonization, which all 3 strains of P. parasiticum were identified using agar block smear and ITS and β-tubulin gene sequencing. All 3 isolates grew initially as white to creamy, yeast-like colonies. After 21 days of incubation at 25 °C, 1 isolate remained light brown, atypical of P. parasiticum. Microscopic examination of agar block smear preparations of all 3 isolates showed thick-walled, medium brown conidiophores that were branched and slightly swollen at the base. The sequences of the ITS and β-tubulin genes of the 3 isolates were identical to those of P. parasiticum. Cases of P. parasiticum infections should be confirmed by a polyphasic approach using morphologic characterization and ITS and β-tubulin gene sequencing.
嗜热拟青霉是一种环境中的暗色有隔霉菌，很少与人类感染有关。我们在此介绍 2 例嗜热拟青霉侵袭性感染病例，包括首例嗜热拟青霉呼吸道感染病例和 1 例气道定植病例，所有 3 株嗜热拟青霉均通过琼脂块涂片和 ITS 和β-微管蛋白基因测序鉴定。所有 3 株分离株最初均为白色至奶油色的酵母样菌落。在 25°C 孵育 21 天后，1 株仍为浅棕色，非嗜热拟青霉典型特征。所有 3 株分离株的琼脂块涂片制备物的显微镜检查显示厚壁、中棕色的分生孢子梗分枝，基部稍肿胀。3 株分离株的 ITS 和β-微管蛋白基因序列与嗜热拟青霉完全一致。应通过形态特征描述和 ITS 和β-微管蛋白基因测序的多相方法来确认嗜热拟青霉感染病例。
