Huang Z H, Graves S, Stewart L, Banks J, Dwyer B
Jiangsu Province Hospital, Nanjing, People's Republic of China.
Comp Immunol Microbiol Infect Dis. 1990;13(3):119-25. doi: 10.1016/0147-9571(90)90274-w.
New endemic areas of spotted fever-like rickettsial disease have been found in south-eastern Australia (Gippsland, Victoria and Flinders Island, Tasmania). The rickettsia responsible is currently unknown although it may be Rickettsia australis. To investigate serological evidence of rickettsial exposure in various wild animal species, a competitive ELISA was developed which detected antibodies to R. australis. It was based on inhibition of an indirect ELISA detecting antibody to R. australis in guinea pig sera. Pre- and post-infection sera from 2 dogs, 2 rabbits, 5 mice and 6 rats, experimentally infected with R. australis, were tested by competitive ELISA. The results showed that all pre-infection sera were negative and all post-infection sera positive for antibody to R. australis. To test the utility of the competitive ELISA for detecting natural rickettsial infection in non-laboratory animals, 51 dog sera, negative for rickettsial antibody by immunofluorescence (IF) and 20 IF positive dog sera (collected from various locations on the east coast of Australia) were tested. Compared to the IF test the competitive ELISA was 90% sensitive and 96% specific. This new test has potential for detecting antibody to R. australis in the sera of different wild animal species.
在澳大利亚东南部（维多利亚州的吉普斯兰以及塔斯马尼亚州的弗林德斯岛）发现了类斑点热立克次体病的新流行地区。尽管致病立克次体可能是澳大利亚立克次体，但目前尚不清楚。为了调查各种野生动物接触立克次体的血清学证据，开发了一种竞争性酶联免疫吸附测定法（ELISA），用于检测针对澳大利亚立克次体的抗体。该方法基于对检测豚鼠血清中澳大利亚立克次体抗体的间接ELISA的抑制作用。用竞争性ELISA检测了2只狗、2只兔子、5只小鼠和6只大鼠经实验感染澳大利亚立克次体后的感染前和感染后血清。结果显示，所有感染前血清对澳大利亚立克次体抗体均呈阴性，所有感染后血清均呈阳性。为了测试竞争性ELISA在检测非实验动物自然立克次体感染中的效用，检测了51份经免疫荧光法（IF）检测立克次体抗体呈阴性的狗血清以及20份IF检测呈阳性的狗血清（从澳大利亚东海岸不同地点采集）。与IF检测相比，竞争性ELISA的灵敏度为90%，特异性为96%。这项新检测方法有潜力检测不同野生动物血清中针对澳大利亚立克次体的抗体。
