Ruffin J, Shaw S, Banerjee S
Department of Biology, North Carolina Central University, Durham 27707.
Cytobios. 1990;63(254-255):167-75.
Several steps were taken to determine the seasonal variation of IgE and IgG antibody against Short Ragweed, Timothy Grass, Tag Alder and White Ash. Extracts of the above pollen grains were separated into their allergen components using SDS-gel electrophoresis and transblotted to nitrocellulose membrane (Western blotting) and probed with sera from atopic patients in every month (August 1987-July 1988). The IgE and IgG antibody against the specific-allergens were detected by double antibody immunoenzyme assay. The percentage of binding was determined by using a 620 video-densitometer. Results indicate that there was no reasonable IgE antibody before the pollination season, but IgEAb appeared after the season and was detectable for several months. Negligible amounts of IgGAb were observed.
采取了几个步骤来确定针对豚草、梯牧草、标签桤木和白蜡树的IgE和IgG抗体的季节性变化。使用SDS-凝胶电泳将上述花粉粒提取物分离成其过敏原成分，然后转印到硝酸纤维素膜上（蛋白质免疫印迹法），并每月用特应性患者的血清进行检测（1987年8月至1988年7月）。通过双抗体免疫酶测定法检测针对特定过敏原的IgE和IgG抗体。结合百分比通过使用620视频密度计来确定。结果表明，在授粉季节之前没有合理的IgE抗体，但IgE抗体在季节之后出现并且在几个月内可检测到。观察到IgG抗体的量可以忽略不计。
