Department of Engineering Sciences, Ångström Laboratory, Uppsala University, Box 534, 751 21 Uppsala, Sweden.
Ultramicroscopy. 2013 Apr;127:48-52. doi: 10.1016/j.ultramic.2012.07.007. Epub 2012 Jul 27.
The early interaction between an implant's surface and bone is a leading factor for implant success, where multiple surface properties contribute to improved bone anchorage. An important parameter is surface topography, both on the micron and nanoscale. Laser-modification has been performed in the thread valleys of Ti6Al4V screws to alter their surface chemistry and topography to form a nanostructured surface titanium-dioxide. Implants were placed in the rabbit tibia, removed with surrounding bone after 8 weeks, fixated, dried and resin embedded. Focused ion beam milling (FIB) was used to prepare specimens from the resin blocks for transmission electron microscopy (TEM). Z-contrast electron tomography offered the possibility to explore the interfacial structure with high-resolution in three-dimensions. With this technique, collagen fibers of the surrounding bone appear to have been laid down parallel to the implant surface. Accordingly, visualization of the laser-modified interface with nanoscale three-dimensional resolution, as offered by Z-contrast electron tomography, gives new insights into bone bonding mechanisms between roughened titanium-dioxide surfaces and bone.
种植体表面与骨早期相互作用是种植体成功的主要因素，其中多种表面特性有助于改善骨锚固。表面形貌是一个重要参数，包括微米和纳米尺度。已经对 Ti6Al4V 螺钉的螺纹槽进行了激光改性，以改变其表面化学性质和形貌，形成纳米结构的二氧化钛表面。将植入物放置在兔胫骨中，8 周后与周围骨骼一起取出，固定，干燥并包埋在树脂中。使用聚焦离子束铣削（FIB）从树脂块中制备用于透射电子显微镜（TEM）的样品。Z 对比度电子断层摄影术提供了用高分辨率在三维空间中探索界面结构的可能性。使用该技术，周围骨的胶原纤维似乎已沿植入物表面平行铺设。因此，Z 对比度电子断层摄影术提供的纳米级三维分辨率的激光改性界面的可视化，为粗糙化二氧化钛表面与骨之间的骨结合机制提供了新的见解。
