Zhou Fei, Yin Ying, Su Ting, Yu Linda, Yu Chang-An
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA.
Biochim Biophys Acta. 2012 Dec;1817(12):2103-9. doi: 10.1016/j.bbabio.2012.08.004. Epub 2012 Aug 19.
The effect of molecular oxygen on the electron transfer activity of the cytochrome bc(1) complex was investigated by determining the activity of the complex under the aerobic and anaerobic conditions. Molecular oxygen increases the activity of Rhodobacter sphaeroides bc(1) complex up to 82%, depending on the intactness of the complex. Since oxygen enhances the reduction rate of heme b(L), but shows no effect on the reduction rate of heme b(H), the effect of oxygen in the electron transfer sequence of the cytochrome bc(1) complex is at the step of heme b(L) reduction during bifurcated oxidation of ubiquinol.
通过测定在需氧和厌氧条件下细胞色素bc(1)复合物的活性，研究了分子氧对该复合物电子传递活性的影响。分子氧可使球形红杆菌bc(1)复合物的活性提高82%，这取决于复合物的完整性。由于氧气提高了血红素b(L)的还原速率，但对血红素b(H)的还原速率没有影响，因此在泛醌分叉氧化过程中，氧气在细胞色素bc(1)复合物电子传递序列中的作用是在血红素b(L)还原步骤。
