CEMAGREF, Hydrosystems and Bioprocesses Unit, Parc de Tourvoie, 92163 Antony, France.
Waste Manag. 2012 Dec;32(12):2258-65. doi: 10.1016/j.wasman.2012.07.024. Epub 2012 Aug 24.
The goal of this study is to evaluate the impact of the inoculum to substrate ratio (I/S) on the anaerobic degradation potential of municipal solid waste (MSW). Reconstituted MSW samples were thus incubated under batch anaerobic conditions and inoculated with an increasing amount of inoculum originating from a mesophilic sludge digester. I/S tested values were 0 (no inoculum added), 0.015, 0.03, 0.06, 0.12, 0.25, 1, 2 and 4 (gVM(inoculum)/gVM(waste)). The results indicate that the apparent maximal rate of dissolved organic carbon accumulation is reached at I/S=0.12. Under this level, the hydrolysis process is limited by the concentration of biomass and can thus be described as first order kinetics phenomena with respect to biomass for I/S ratios below 0.12. The maximum methane production rate and the minimal latency are reached at a ratio of 2. In addition to that, both methane signature and ARISA show a shift in the methanogenic populations from hydrogenotrophic to acetoclastic.
本研究旨在评估接种物与底物比（I/S）对城市固体废物（MSW）厌氧降解潜力的影响。因此，将再生物质的 MSW 样品在分批厌氧条件下进行孵育，并接种一定量的来自中温污泥消化器的接种物。测试的 I/S 值为 0（未添加接种物）、0.015、0.03、0.06、0.12、0.25、1、2 和 4（gVM（接种物）/gVM（废物））。结果表明，溶解有机碳积累的表观最大速率在 I/S=0.12 时达到。在这个水平下，水解过程受到生物量浓度的限制，因此可以描述为低于 0.12 的 I/S 比下的生物质一级动力学现象。最大甲烷生成速率和最小潜伏期在 2 的比例下达到。此外，甲烷特征和 ARISA 均表明产甲烷种群从氢营养型向乙酸营养型转移。
