Department of Animal Pathology, Obstetrics and Reproduction Area, Faculty of Veterinary, University of Zaragoza, Zaragoza, Spain.
Vet Rec. 2012 Sep 22;171(12):298. doi: 10.1136/vr.100579. Epub 2012 Aug 24.
Changes in the genital mucus around the oestrus are used by different diagnostic methods to determine optimal fertilisation time. In the current study, the authors evaluated the different arborisation patterns found in vestibular mucus, and also established its relationship with vestibular resistance changes during oestrus. Thirty multiparous sows were checked by transrectal ultrasonography to determine ovulation time every 12 hours. Vestibular resistance was measured with a commercial resistance probe, and vestibular mucus ferning was also evaluated every 12 hours during the oestrus. Significant changes (P < 0.05) in vestibular resistance were detected, registering high variation among individuals. Maximum resistance data was reached between 12 and 24 hours after ovulation time in 83 per cent of the sows. Crystallisation samples were classified into three different patterns according to the fern-like crystal degree. Arborisation peak occurred from 48 to 36 hours before the moment of ovulation, when vestibular resistance values increased gradually. In the optimal insemination moment, vestibular resistance increased significantly (P < 0.05) and vestibular mucus showed a low crystallisation pattern (P < 0.05). Combining several methods to measure genital mucus changes may predict the ovulation time and the best insemination moment.
发情期生殖道黏液的变化可通过不同的诊断方法来确定最佳受精时间。在本研究中，作者评估了前庭黏液中发现的不同分支模式，并确定了其与发情期前庭阻力变化的关系。30 头经产母猪通过直肠超声检查每 12 小时确定一次排卵时间。使用商业阻力探头测量前庭阻力，并且在发情期每 12 小时评估前庭黏液的羊齿状结晶。检测到前庭阻力的显著变化（P<0.05），个体之间存在很大的差异。在 83%的母猪中，排卵后 12 至 24 小时达到最大阻力数据。结晶样本根据羊齿状晶体程度分为三种不同的模式。分支峰值出现在排卵前 48 至 36 小时，此时前庭阻力值逐渐增加。在最佳输精时刻，前庭阻力显著增加（P<0.05），前庭黏液显示出低结晶模式（P<0.05）。结合多种方法测量生殖道黏液的变化可以预测排卵时间和最佳输精时刻。
