Chiba M
Division of Medical Life Sciences, Department of Biomedical Sciences, Hirosaki University Graduate School of Health Sciences, 66-1 Hon-cho, Hirosaki, Aomori 036-8564, Japan.
Radiat Prot Dosimetry. 2012 Nov;152(1-3):164-7. doi: 10.1093/rpd/ncs216. Epub 2012 Aug 23.
Ionising radiation (IR) causes DNA (deoxyribonucleic acid) injury and activates intracellular signal pathways including the regulation of DNA repair and cell cycle. However, the further knowledge of molecular events involved in radiation exposure is essential to more comprehensively understand the effects of irradiation. Therefore, the gene expressions of mRNA (messenger ribonucleic acid) by X-ray irradiation in human B lymphoblast cell line (IM-9) using a microarray were investigated. The mRNA expressions of 65 genes were shown to be up-regulated at >2.0-fold in irradiated cells (4 Gy) when compared with non-irradiated cells (0 Gy) by microarray analysis. Among 65 genes, a large number of genes were up-regulated with an X-ray dose-dependent change. These results indicate that the up-regulation of their mRNAs is the effects of irradiation and may be due to biological dosimetric markers for the evaluation of radiation exposure in the future.
电离辐射（IR）会导致DNA（脱氧核糖核酸）损伤，并激活包括DNA修复和细胞周期调控在内的细胞内信号通路。然而，进一步了解辐射暴露所涉及的分子事件对于更全面地理解辐射的影响至关重要。因此，我们使用微阵列研究了X射线照射对人B淋巴母细胞系（IM-9）中mRNA（信使核糖核酸）基因表达的影响。通过微阵列分析，与未照射细胞（0 Gy）相比，照射细胞（4 Gy）中65个基因的mRNA表达上调超过了2.0倍。在这65个基因中，大量基因随着X射线剂量的变化而呈剂量依赖性上调。这些结果表明，它们的mRNA上调是辐射的影响，未来可能作为评估辐射暴露的生物剂量学标志物。
