Department of Pathology, Tsukuba University Hospital, Ibaraki, Japan.
Pathol Int. 2012 Sep;62(9):592-9. doi: 10.1111/j.1440-1827.2012.02847.x.
Whole-slide imaging (WSI) has been used for education and histological image preservation, and several studies have also reported its validity for practical pathological diagnosis. However, such studies employed materials stained with hematoxylin-eosin (HE), and very few attempts have been made to use immunohistochemically stained materials for diagnostic purposes. In the present study, we investigated the availability of WSI diagnosis for immunohistochemically stained materials in place of routine glass slides. Thirty pathologists participated in a trial of HER2 expression diagnosis using WSI and compared the results with those obtained by light microscopy. The validity of WSI diagnosis (interobserver agreement) was rated as 'substantial' in comparison with glass slide diagnosis (κ-value = 0.719). There was a tendency for observers to assign higher scores with WSI than with glass slides, probably because WSI requires slides to be scanned into a computer and observed via a monitor. Although we were able to demonstrate the potential utility of WSI for diagnosing immunostained materials, it must be borne in mind that there are some differences in visualization between WSI and glass slides.
全切片成像（WSI）已被用于教育和组织学图像保存，并且已经有几项研究报告了其在实际病理诊断中的有效性。然而，这些研究使用的是经苏木精-伊红（HE）染色的材料，很少有尝试使用免疫组织化学染色材料进行诊断。在本研究中，我们研究了用 WSI 替代常规玻璃载玻片进行免疫组织化学染色材料诊断的可行性。30 名病理学家参与了使用 WSI 进行 HER2 表达诊断的试验，并将结果与通过光学显微镜获得的结果进行了比较。与玻璃载玻片诊断相比，WSI 诊断的有效性（观察者间一致性）被评为“中等”（κ 值=0.719）。观察者倾向于使用 WSI 分配更高的分数，这可能是因为 WSI 需要将幻灯片扫描到计算机中，并通过显示器进行观察。虽然我们能够证明 WSI 诊断免疫染色材料的潜在效用，但必须注意到 WSI 和玻璃载玻片之间在可视化方面存在一些差异。
