Departamento de Química Analítica y Tecnología de los Alimentos, Facultad de Ciencias Ambientales y Bioquímica, Universitat de Castilla-La Mancha, Toledo, Spain.
J Food Sci. 2012 Oct;77(10):M579-85. doi: 10.1111/j.1750-3841.2012.02895.x. Epub 2012 Aug 27.
Different wine varieties, including some with low pH, were studied to determine the ability to grow and produce secondary metabolites of a previously selected autochthonous Oenococcus oeni strain (C22L9), compared with a commercial strain. Monitoring of malolactic fermentation (MLF) was carried out by microbiological and chemical analysis of wines. The concentration of some major volatile compounds and biogenic amines in wines before and after malolactic fermentation was also determined. The results showed major differences in MLF duration both between wines and strains, although the differences between strains were slight for most of the analyzed compounds. Statistically significant differences in citric acid degradation were found in all wine varieties and it was confirmed that O. oeni C22L9 is a poor degrader of citric acid; this means that MLF can be prolonged without the risk of producing high concentrations of diacetyl and acetoin. Sensory analysis of wines after MLF showed similar characteristics in wines from both strains. This study thus shows that O. oeni C22L9 possesses even better sensory and fermentation properties than the commercial strain and can be used in wines with different characteristics, which makes it highly valuable for industrial use.
The increasingly use of grape varieties of low pH in winemaking and the higher alcohol content of wines, as a consequence of the climatic change, make interesting the study of the behavior during MLF of O. oeni strains in order to determine their ability to grow, when growth conditions are not optimal, and to produce secondary metabolites. A comparative study was conducted using an autochthonous O. oeni strain (C22L9) and a commercial O. oeni strain and 4 wine varieties.
研究了不同的葡萄酒品种,包括一些 pH 值较低的品种,以确定先前选择的本土酒酒球菌(C22L9)菌株与商业菌株相比生长和产生次生代谢物的能力。通过微生物学和化学分析监测葡萄酒的苹果酸-乳酸发酵(MLF)。还测定了 MLF 前后葡萄酒中一些主要挥发性化合物和生物胺的浓度。结果表明,MLF 持续时间在葡萄酒和菌株之间存在很大差异,尽管对于大多数分析化合物,菌株之间的差异很小。在所有葡萄酒品种中均发现柠檬酸降解存在统计学上的显著差异,并证实 O. oeni C22L9 是柠檬酸的不良降解菌;这意味着 MLF 可以延长而不会产生高浓度的双乙酰和乙酰基。MLF 后葡萄酒的感官分析显示,两种菌株的葡萄酒具有相似的特征。因此,本研究表明,O. oeni C22L9 具有比商业菌株更好的感官和发酵特性,可用于具有不同特性的葡萄酒,这使其在工业用途中具有很高的价值。
由于气候变化,酿酒中低 pH 值葡萄品种的使用越来越多,以及葡萄酒的酒精含量较高,因此研究 O. oeni 菌株在 MLF 过程中的行为很有趣,以确定它们在生长条件不理想时的生长能力,以及产生次生代谢物的能力。使用一株本土 O. oeni 菌株(C22L9)和一株商业 O. oeni 菌株以及 4 个葡萄酒品种进行了比较研究。