Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam, India.
Vector Borne Zoonotic Dis. 2012 Nov;12(11):953-60. doi: 10.1089/vbz.2011.0948. Epub 2012 Aug 27.
The current work focuses on the study of polymeric, biodegradable nanoparticles (NPs) for the encapsulation of doxorubicin and mitomycin C (anti-leishmanial drugs), and their efficient delivery to macrophages, the parasite's home. The biodegradable polymer methoxypoly-(ethylene glycol)-b-poly (lactic acid) (MPEG-PLA) was used to prepare polymeric NPs encapsulating doxorubicin and mitomycin C. The morphology, mean diameter, and surface area of spherical NPs were determined by transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), and BET surface area analysis. X-ray diffraction was performed to validate drug encapsulation. An in vitro release profile of the drugs suggested a fairly slow release. These polymeric NPs were efficiently capable of releasing drug inside macrophages at a slower pace than the free drug, which was monitored by epi-fluorescence microscopy. Encapsulation of doxorubicin and mitomycin C into NPs also decreases cellular toxicity in mouse macrophages (J774.1A).
目前的工作重点是研究用于封装多柔比星和丝裂霉素 C（抗利什曼原虫药物）的聚合、可生物降解的纳米颗粒 (NPs)，并将其有效地递送至寄生虫的家园——巨噬细胞。使用可生物降解的聚合物甲氧基聚（乙二醇）-b-聚（乳酸）（MPEG-PLA）来制备封装多柔比星和丝裂霉素 C 的聚合 NPs。通过透射电子显微镜 (TEM)、场发射扫描电子显微镜 (FESEM) 和 BET 表面积分析确定了球形 NPs 的形态、平均直径和表面积。X 射线衍射用于验证药物包封。药物的体外释放曲线表明释放速度相当缓慢。这些聚合 NPs 能够以比游离药物更慢的速度在巨噬细胞内有效地释放药物，这可以通过荧光显微镜监测到。将多柔比星和丝裂霉素 C 包封到 NPs 中也降低了小鼠巨噬细胞 (J774.1A) 的细胞毒性。
