腺病毒2型DNA 5'末端的55K蛋白与病毒编码的候选转化蛋白（E1-53K、E1-40K-50K）和DNA结合蛋白（E2-42K/47K/73K）无关。

The 55K protein on the 5' termini of adenovirus type 2 DNA is unrelated to virus-coded candidate transformation proteins (E1-53K, E1-40K-50K) and DNA-binding proteins (E2-42K/47K/73K).

作者信息

Green M, Wold W S, Brackmann K H, Cartas M A

出版信息

J Virol. 1979 Sep;31(3):836-40. doi: 10.1128/JVI.31.3.836-840.1979.

DOI:10.1128/JVI.31.3.836-840.1979

PMID:229254

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC353511/

Abstract

A polypeptide of 55,000 daltons (55K) is linked, probably covalently, to the K' termini of adenovirus type 2 DNA. The 55K polypeptide is synthesized during early stages of infection (T. Yamashita, M. Arens, and M. Green, J. Virol. 30: 497-507, 1979) and thus may function in viral DNA replication, gene regulation, or cell transformation. Several virus-coded early polypeptides have been identified that could correspond to the terminal 55K, including the E1-40K-50K and E1-53K candidate transformation polypeptides and the E2-42K/47K/73K single-stranded DNA-binding polypeptide. We show here that two-dimensional tryptic [35S]methionine-peptide maps of the terminal 55K differ completely from [35S]methionine-peptide maps of four related E1-40K-50K polypeptides, the E1-53K, and the related E2-42K, E2-47K, and E2-73K polypeptides. We conclude that the terminal 55K polypeptide does not correspond to any of the known virus-coded early polypeptides.

摘要

一种55000道尔顿（55K）的多肽可能以共价键的形式与2型腺病毒DNA的K′末端相连。55K多肽是在感染早期合成的（T. 山下、M. 阿伦斯和M. 格林，《病毒学杂志》30: 497 - 507, 1979），因此可能在病毒DNA复制、基因调控或细胞转化中发挥作用。已经鉴定出几种病毒编码的早期多肽，它们可能与末端的55K相对应，包括E1 - 40K - 50K和E1 - 53K候选转化多肽以及E2 - 42K/47K/73K单链DNA结合多肽。我们在此表明，末端55K的二维胰蛋白酶[35S]甲硫氨酸肽图谱与四种相关的E1 - 40K - 50K多肽、E1 - 53K以及相关的E2 - 42K、E2 - 47K和E2 - 73K多肽的[35S]甲硫氨酸肽图谱完全不同。我们得出结论，末端55K多肽与任何已知的病毒编码早期多肽都不对应。