Center of Excellence in Electrochemistry, University of Tehran, Tehran, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2012 Oct;96:801-4. doi: 10.1016/j.saa.2012.07.088. Epub 2012 Aug 3.
In this work, L-cysteine capped-ZnS quantum dots were synthesized in aqueous medium, and their interaction with some of the amino acids was studied with fluorescence spectra. The results demonstrated that histidine could effectively quench the Quantum dots emission more than other amino acids. Electron transfer process between the capping ligands and histidine was mainly responsible for the remarkable quenching effect of histidine, because according to the structure of histidine, it is the strongest acceptor among essential amino acids. Under optimum conditions, the quenched fluorescence intensity increased linearly with the concentration of histidine ranging from 1.33×10(-6) to 1.49×10(-4) mol L(-1). The limit of detection for histidine was 0.05×10(-7) mol L(-1).
在这项工作中，我们在水相介质中合成了半胱氨酸封端的 ZnS 量子点，并通过荧光光谱研究了它们与一些氨基酸的相互作用。结果表明，组氨酸比其他氨基酸更能有效地猝灭量子点的发射。配体和组氨酸之间的电子转移过程是组氨酸显著猝灭效应的主要原因，因为根据组氨酸的结构，它是必需氨基酸中最强的受体。在最佳条件下，猝灭荧光强度随组氨酸浓度在 1.33×10(-6)至 1.49×10(-4)mol/L 范围内呈线性增加。组氨酸的检测限为 0.05×10(-7)mol/L。
