Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Jan;78(1):537-42. doi: 10.1016/j.saa.2010.11.023. Epub 2010 Nov 26.
Aqueous polymethylmethacrylate (PMMA)-capped CdSe/ZnS quantum dots were used as fluorescence probes for paeonol determination. Based on the fluorescence quenching of aqueous CdSe/ZnS quantum dots caused by paeonol, a simple, sensitive and rapid method was developed. Under the optimal conditions, with excitation and emission wavelengths at 350 nm and 620 nm, respectively, the calibration plot of F0-F with concentration of paeonol was linear in the range of 25.04-175.2 mg L(-1) with correlation coefficient of 0.9986. The limit of detection was 0.017 mg L(-1). The concentration of paeonol in paeonol ointment was determined by the proposed method and the result agreed with the claimed value. Furthermore, the possible fluorescence quenching mechanism was discussed.
水相聚甲基丙烯酸甲酯（PMMA）封端的CdSe/ZnS 量子点被用作丹皮酚测定的荧光探针。基于丹皮酚对水相 CdSe/ZnS 量子点荧光的猝灭作用，建立了一种简单、灵敏、快速的方法。在最佳条件下，激发和发射波长分别为 350nm 和 620nm，丹皮酚浓度在 25.04-175.2mg/L 范围内与（F0-F）的线性关系良好，相关系数为 0.9986。检出限为 0.017mg/L。采用所提出的方法测定丹皮酚软膏中丹皮酚的浓度，结果与标示量相符。此外，还讨论了可能的荧光猝灭机制。
