Department of Biochemistry and Molecular Biology, The Brody School of Medicine at East Carolina University, Greenville, NC 27834, USA.
J Nutr Biochem. 2013 May;24(5):832-41. doi: 10.1016/j.jnutbio.2012.05.001. Epub 2012 Aug 25.
Retinoids are essential in the proper establishment and maintenance of immunity. Although retinoids are implicated in immune related processes, their role in immune cell adhesion has not been well established. In this study, the effect of 9-cis-retinoic acid (9-cis-RA) on human hematopoietic cell adhesion was investigated. 9-cis-RA treatment specifically induced cell adhesion of the human immune cell lines HuT-78, NB4, RPMI 8866 and U937. Due to the prominent role of integrin receptors in mediating immune cell adhesion, we sought to evaluate if cell adhesion was integrin-dependent. By employing a variety of integrin antagonist including function-blocking antibodies and EDTA, we establish that 9-cis-RA prompts immune cell adhesion through established integrin receptors in addition to a novel integrin-independent process. The novel integrin-independent adhesion required the presence of retinoid and was attenuated by treatment with synthetic corticosteroids. Finally, we demonstrate that 9-cis-RA treatment of primary murine B-cells induces ex vivo adhesion that persists in the absence of integrin function. Our study is the first to demonstrate that 9-cis-RA influences immune cell adhesion through at least two functionally distinct mechanisms.
视黄酸在适当的免疫建立和维持中是必不可少的。尽管视黄酸参与免疫相关过程，但它们在免疫细胞黏附中的作用尚未得到很好的确立。在这项研究中，研究了 9-顺式视黄酸（9-cis-RA）对人造血细胞黏附的影响。9-cis-RA 处理特异性诱导人免疫细胞系 HuT-78、NB4、RPMI 8866 和 U937 的细胞黏附。由于整合素受体在介导免疫细胞黏附中的突出作用，我们试图评估细胞黏附是否依赖于整合素。通过使用多种整合素拮抗剂，包括功能阻断抗体和 EDTA，我们确定 9-cis-RA 通过除了新的整合素非依赖性过程之外的已建立的整合素受体来促进免疫细胞黏附。新型整合素非依赖性黏附需要视黄酸的存在，并被合成皮质类固醇处理所减弱。最后，我们证明 9-cis-RA 处理原代小鼠 B 细胞诱导体外黏附，在没有整合素功能的情况下仍能持续存在。我们的研究首次表明，9-cis-RA 通过至少两种功能不同的机制影响免疫细胞黏附。
