Department of Hepatobiliary & Pancreatic Diseases, School of Medicine, Shanghai East Hospital, Tongji University, Shanghai 200120, China.
Molecules. 2012 Aug 27;17(9):10267-75. doi: 10.3390/molecules170910267.
Oxidative stress is involved in the development and progression of disease. Because sodium aescinate has been reported to have immunity enhancing and antioxidative effects, we investigated its activity by employing a hepatocellular carcinoma (HCC) mouse model. Sixty BALB/c mice were randomly divided into four groups, including a 1.4 mg/kg treated group (n = 15), a 2.8 mg/kg treated group (n = 15), an untreated hepatocellular carcinoma control group (n = 15) and a normal control group (n = 15). After H22 cells were cultured for one week, we collected 2 × 10⁶ cells and injected them subcutaneously as 0.2 mL cell suspensions in sterile saline into the right shoulder region of every mouse. The animals were monitored for changes in activity, physical condition and body weight during the experiment. The next day after injection of H22 cells, animals in these test groups received one intraperitoneal injection of drug or physiological saline for 13 days. Results showed that in the sodium aescinate injection liquid (SAIL)-treated HCC mice, serum interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), Gamma-glutamyltransferase (γ-GT), alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) levels were significantly decreased compared with normal control mice. In addition, treatment with sodium aescinate injection liquid significantly decreased blood and liver malondialdehyde (MDA) levels, increased glutathione (GSH) levels, and antioxidant enzyme [superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px)] activities in a dose-dependent manner. We conclude that sodium aescinate injection liquid can decrease oxidative injury and enhance immunity functions in HCC mice.
氧化应激参与疾病的发生和发展。由于已报道七叶皂苷钠具有增强免疫和抗氧化作用，我们采用肝癌（HCC）小鼠模型来研究其活性。将 60 只 BALB/c 小鼠随机分为四组，包括 1.4mg/kg 治疗组（n=15）、2.8mg/kg 治疗组（n=15）、未经治疗的肝癌对照组（n=15）和正常对照组（n=15）。将 H22 细胞培养一周后，收集 2×106 个细胞，作为 0.2mL 细胞悬浮液在无菌盐水中注射到每只小鼠的右肩区域。在实验过程中，监测动物的活动、身体状况和体重变化。H22 细胞注射后的第二天，这些测试组的动物接受一次腹腔内注射药物或生理盐水，共 13 天。结果表明，在七叶皂苷钠注射液（SAIL）治疗的 HCC 小鼠中，血清白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）、γ-谷氨酰转移酶（γ-GT）、丙氨酸转氨酶（ALT）、天门冬氨酸转氨酶（AST）和碱性磷酸酶（ALP）水平与正常对照组小鼠相比显著降低。此外，七叶皂苷钠注射液治疗还显著降低了血液和肝脏丙二醛（MDA）水平，增加了谷胱甘肽（GSH）水平，并呈剂量依赖性地增加了抗氧化酶[超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）]的活性。我们得出结论，七叶皂苷钠注射液可降低 HCC 小鼠的氧化损伤并增强其免疫功能。
