State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
Gen Comp Endocrinol. 2012 Nov 1;179(2):159-66. doi: 10.1016/j.ygcen.2012.08.010. Epub 2012 Aug 19.
Neuropeptide Y (NPY) is a member of the pancreatic polypeptide family which is a potent orexigenic peptide known to date in mammals and teleost. This study was carried out to investigate the effects of NPY on food intake and growth of orange-spotted grouper (Epinephelus coioides). Synthetic grouper NPY (gNPY) was given orally at the dose of 0.5, 1.0 and 2.0 μg/g feed for 50 days, results showed that NPY treatment (1.0 and 2.0 μg/g feed) significantly increased growth rate, weight gain, feed conversion efficiency (FCE) and pituitary growth hormone (GH) mRNA level than the control group (p<0.05). Furthermore, high level secretion of gNPY was expressed and purified in the Pichia pastoris expression system. The bioactivity of recombinant gNPY was confirmed by its ability to up-regulate GH mRNA expression in vivo and in vitro and down-regulate preprosomatostatin I (PSSI) mRNA expression in vivo. These results demonstrate that NPY has stimulatory effects on food intake as well as growth of grouper as in other teleost fish, also indicate that recombinant gNPY from P. pastoris has the same bioactivity as synthetic gNPY and has the potential to be used as a feed additive for both research and aquatic application.
神经肽 Y（NPY）是胰多肽家族的一员，是迄今为止在哺乳动物和硬骨鱼中发现的一种有效的食欲肽。本研究旨在研究 NPY 对橙点石斑鱼（Epinephelus coioides）摄食和生长的影响。合成的石斑鱼 NPY（gNPY）以 0.5、1.0 和 2.0μg/g 饲料的剂量口服给予 50 天，结果表明，NPY 处理（1.0 和 2.0μg/g 饲料）与对照组相比，显著提高了生长率、体重增加、饲料转化率（FCE）和垂体生长激素（GH）mRNA 水平（p<0.05）。此外，在毕赤酵母表达系统中表达和纯化了高表达水平的 gNPY。重组 gNPY 的生物活性通过其在体内和体外上调 GH mRNA 表达以及在体内下调前体生长抑素 I（PSSI）mRNA 表达的能力得到证实。这些结果表明，NPY 对石斑鱼的摄食和生长具有刺激作用，与其他硬骨鱼一样，也表明毕赤酵母中的重组 gNPY 具有与合成 gNPY 相同的生物活性，并有潜力作为研究和水产应用的饲料添加剂。
