采用聚合酶链反应检测单纯乙肝核心抗体阳性献血者的HBV DNA。
The detection of HBV DNA with polymerase chain reaction in blood donors with isolated hepatitis B core antibody.
作者信息
Tas Tekin, Kaya Selcuk, Onal Suleyman, Kucukbayrak Abdulkadir
机构信息
Department of Medical Microbiology, School of Medicine, Abant Izzet Baysal University, Bolu, Turkey.
出版信息
Med Glas (Zenica). 2012 Aug;9(2):227-30.
AIM
To analyze the presence of HBV DNA in blood donors admitted to blood banks with HBsAg negativity and isolated anti-HBc positivity.
METHODS
Sera samples of 2500 HBsAg negative donors were included in the study. HBsAg tests were assayed with VITROS analyzer. Anti-HBc total, anti-HBs, HBeAg, anti-HBe and anti-HBc IgM tests were manually studied with DIA PRO kit by employing ELISA method. HBV DNA test was evaluated with iQ5 Real Time PCR Detection System with real-time PCR method.
RESULTS
A total number of 401 (16.4%) HBsAg negative blood donors had anti-HBc positivity. Forty-five of 401 (1.8%) anti-HBc positive samples were anti-HBs negative. These 45 persons were evaluated for anti-HBc positivity. Thirty-six of 45 anti-HBc positive persons had only anti-HBc positivity, and other nine patients had Anti-HBe antibody. HBV DNA was detected in three (6.6 %) of 45 isolated Anti-HBc positive persons as in one of nine persons and two of 36 persons.
CONCLUSION
The persons who have only HBs Ag negativity may not be appropriate to become blood donors. Anti-HBc test should be done for HBsAg negative persons. They should not be accepted as blood donors if Anti-HBc is found positive.
目的
分析乙肝表面抗原（HBsAg）阴性且单独抗-HBc阳性的献血者血液中乙肝病毒脱氧核糖核酸（HBV DNA）的存在情况。
方法
2500名HBsAg阴性献血者的血清样本纳入研究。使用VITROS分析仪检测HBsAg。采用酶联免疫吸附测定（ELISA）法，使用DIA PRO试剂盒手动检测抗-HBc总量、抗-HBs、HBeAg、抗-HBe和抗-HBc IgM。采用实时荧光定量聚合酶链反应（PCR）方法，使用iQ5实时荧光定量PCR检测系统评估HBV DNA检测。
结果
共有401名（16.4%）HBsAg阴性献血者抗-HBc呈阳性。401份抗-HBc阳性样本中有45份（1.8%）抗-HBs呈阴性。对这45人进行抗-HBc阳性评估。45名抗-HBc阳性者中有36人仅抗-HBc呈阳性，其他9名患者有抗-HBe抗体。45名单独抗-HBc阳性者中有3名（6.6%）检测到HBV DNA，其中9人中有1人，36人中有2人。
结论
仅HBsAg阴性的人可能不适合成为献血者。HBsAg阴性者应进行抗-HBc检测。如果发现抗-HBc呈阳性，则不应接受其作为献血者。
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