The Key Laboratory for Silviculture and Conservation of Ministry of Education, College of Forestry, Beijing Forestry University, Beijing 100083, People's Republic of China.
Microsc Res Tech. 2012 Dec;75(12):1700-10. doi: 10.1002/jemt.22118. Epub 2012 Aug 28.
The morphology and ultrastructure of the antennae and antennal sensilla of seven male Dendrolimus species and a male Trabala vishnou gigantina (Yang) (Lepidoptera: Lasiocampidae) were examined by light microscope and scanning electron microscope. Six morphological types of antennal sensilla were identified: Sensilla trichodea, Sensilla chaetica, Sensilla styloconica, Sensilla coeloconica, Böhm bristles, and foot-like sensilla. Six of the Dendrolimus moths and Trabala vishnou gigantina Yang share the same antennal sensilla type, as do various geographic populations of the same species. The exception, Dendrolimus spectabilis Butler, has foot-like sensilla. However, the antennal sensilla subtypes were significantly different among species and/or populations. There were no remarkable differences in the width of the scape, pedicel, subflagellum, and the side-branches between the eight male species studied. However, we observed significant differences in the number of flagellomere and the length of scape, pedicel, subflagellums, and side-branches. The length and basal diameter of various types of antennal sensilla did not vary significantly among Dendrolimus moths. Beyond that, there were no differences among populations of the same kind of species. Hierarchical cluster analysis found two clusters: the first contained D. punctata punctata (Walker), D. punctata wenshanensis (Tsai et Liu), D. tabulaeformis (Tsai et Liu), and D. spectabilis and D. superans (Butler), and the second contained D. grisea (Moore) and D. kikuchii kikuchii (Matsumura). Trabala vishnou gigantina was placed separately from the two clusters. We conclude that D. punctata wenshanensis,D. tabulaeformis, and D. spectabilis are geographic subspecies of D. punctata punctata.
通过光学显微镜和扫描电子显微镜观察了 7 种雄性马尾松毛虫和 1 种雄性云南松毛虫（鳞翅目：枯叶蛾科）的触角形态和超微结构。鉴定出 6 种形态类型的触角感器：毛形感器、刺形感器、锥形感器、腔锥形感器、Böhm 鬃毛和附足形感器。其中 6 种马尾松毛虫和云南松毛虫与各种地理种群的同一物种具有相同的触角感器类型。例外的是，马尾松毛虫有附足形感器。然而，种间和/或种群间的触角感器亚型存在显著差异。在所研究的 8 种雄性物种中，触角柄、柄节、梗节和侧支的宽度没有明显差异。然而，我们观察到触角鞭节的节数和柄节、柄节、梗节和侧支的长度存在显著差异。各种类型的触角感器的长度和基部直径在马尾松毛虫中没有显著差异。此外，同一物种的种群之间没有差异。层次聚类分析发现 2 个聚类：第一个聚类包含马尾松毛虫指名亚种、文山松毛虫、云南松毛虫和马尾松毛虫和云南松毛虫，第二个聚类包含油松毛虫和云南松毛虫。云南松毛虫被单独放置在这两个聚类之外。我们得出结论，文山松毛虫、云南松毛虫和马尾松毛虫是马尾松毛虫的地理亚种。
