Magill Paul, Walsh Pauline, Murphy Terence, Mulhall Kevin J
J Orthop Sci. 2012 Nov;17(6):802-7. doi: 10.1007/s00776-012-0295-8. Epub 2012 Aug 29.
The ischaemia-reperfusion (IR) injury causes significant morbidity. Ischaemic preconditioning (IPC) is a technique for limiting the effects of the IR injury. Its potential has not yet been harnessed in orthopaedics.
To establish a novel in vitro IR model using a human skeletal muscle cell line. Secondly, to introduce simulated IPC to the model and examine the effect of this on cell viability.
A human skeletal muscle cell line was cultured in vitro. Placing the cells in a hypoxic buffer and a closed hypoxic environment simulated ischaemia. Reversing this process simulated reperfusion. IPC was simulated by alternate cycles of ischaemia and reperfusion. Cell viability comparisons were made between control and experimental groups of cells.
A reproducible in vitro IR model was established. The addition of simulated IPC is associated with increased cell death at 12 and 24 h of reperfusion. Significantly greater cell survival is seen in the IPC group when measured at 72 h reperfusion.
We hypothesise that IPC initially decreases cell number. The remaining cells are more robust. This selected cell line then expands over the course of 72 h and displays greater resistance to the IR injury. This theory can help explain delayed preconditioning.
缺血再灌注（IR）损伤会导致严重的发病情况。缺血预处理（IPC）是一种限制IR损伤影响的技术。其潜力尚未在骨科领域得到利用。
使用人骨骼肌细胞系建立一种新型的体外IR模型。其次，将模拟IPC引入该模型并检查其对细胞活力的影响。
在体外培养人骨骼肌细胞系。将细胞置于缺氧缓冲液和封闭的缺氧环境中模拟缺血。逆转此过程模拟再灌注。通过缺血和再灌注的交替循环模拟IPC。对对照组和实验组细胞进行细胞活力比较。
建立了可重复的体外IR模型。在再灌注12小时和24小时时，添加模拟IPC与细胞死亡增加相关。在再灌注72小时时测量，IPC组的细胞存活率明显更高。
我们假设IPC最初会减少细胞数量。剩余的细胞更具活力。然后这种选定的细胞系在72小时的过程中扩增，并对IR损伤表现出更大的抵抗力。这一理论有助于解释延迟预处理。
