Persohn E, Schachner M
Hoffman-La Roche Ltd, Basel, Switzerland.
J Neurocytol. 1990 Dec;19(6):807-19. doi: 10.1007/BF01186812.
The expression of the neural cell adhesion molecules L1 and N-CAM was investigated in developing postnatal mouse hippocampus by immunocytochemical techniques at the light and electron microscopic levels. In the 1, 8 and 21-day-old hippocampus, L1 was only observed on fasciculating axons. L1 was not detectable on dendrites and cell bodies of pyramidal cells, granule cells and interneurons in any of the hippocampal regions studied. Also, synapses were never found to be L1-immunoreactive either pre- or postsynaptically. L1 was not detectable at contacts between astrocytes and axons. Polyclonal N-CAM antibodies reacting with the three components of N-CAM (N-CAM total) stained all neuronal and glial cell types in the 1, 8 and 21-day-old hippocampus. In contrast, the 180 kDa component of N-CAM (N-CAM 180) was only detectable on neuronal cell bodies and dendrites of the 1 and 8-day-old hippocampus. In the 21-day-old hippocampus N-CAM 180 was not recognized on neuronal cell bodies. N-CAM 180 was strongly expressed in postsynaptic sites at all ages studies. We infer from these observations that L1 is predominantly involved in axon fasciculation, whereas N-CAM 180 appears to be more characteristic of stabilizing cell contacts, particularly at the synapse.
采用免疫细胞化学技术，在光学显微镜和电子显微镜水平上，对出生后发育阶段的小鼠海马体中神经细胞黏附分子L1和N-钙黏蛋白（N-CAM）的表达进行了研究。在出生1天、8天和21天的海马体中，仅在成束的轴突上观察到L1。在所研究的任何海马区域的锥体细胞﹑颗粒细胞和中间神经元的树突及细胞体上均未检测到L1。此外，在突触前或突触后均未发现L1免疫反应性的突触。在星形胶质细胞与轴突的接触部位未检测到L1。与N-CAM的三种成分发生反应的多克隆N-CAM抗体（N-CAM总量），对出生1天、8天和21天的海马体中的所有神经元和神经胶质细胞类型进行了染色。相比之下，N-CAM的180 kDa成分（N-CAM 180）仅在出生1天和8天的海马体的神经元细胞体和树突上可检测到。在出生21天的海马体中，未在神经元细胞体上识别出N-CAM 180。在所有研究的年龄段中，N-CAM 180在突触后部位均有强烈表达。从这些观察结果中我们推断，L1主要参与轴突成束，而N-CAM 180似乎更具稳定细胞接触的特征，尤其是在突触处。
