School of Interdisciplinary Bioscience and Bioengineering I-Bio, Pohang University of Science and Technology POSTECH, Pohang, Gyeongbuk, South Korea.
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2013 Jan-Feb;5(1):67-74. doi: 10.1002/wnan.1182. Epub 2012 Aug 23.
Immunological synapse (IS) is a complex supramolecular structure formed at the interface between T cells and antigen presenting cells (APCs) during T cell antigen recognition. Microfabricated platforms have made great contributions to our understanding of the assembly dynamics and functional roles of the T cell synapses over the last decade. Here, we review three different types of microfabricated platforms developed to modulate and monitor the T cell synapse assembly. Firstly, multi-protein micropatterned surfaces presenting key ligands for T cell activation that can modulate the spatial distribution of receptors/signaling molecules in T cell synapses are described. Secondly, micropatterned supported bilayers that can modulate the dynamics of T cell receptor (TCR) microclusters are introduced. Lastly, T-APC pair arrays that allow for improved fluorescence live cell imaging are discussed.
免疫突触（IS）是 T 细胞与抗原呈递细胞（APC）在 T 细胞抗原识别过程中，在细胞间界面形成的一种复杂的超分子结构。在过去的十年中，微制造平台为我们理解 T 细胞突触的组装动力学和功能作用做出了巨大贡献。在这里，我们综述了三种不同类型的微制造平台，用于调节和监测 T 细胞突触的组装。首先，描述了展示用于 T 细胞激活的关键配体的多蛋白微图案表面，这些配体可以调节 T 细胞突触中受体/信号分子的空间分布。其次，介绍了可以调节 T 细胞受体（TCR）微簇动力学的图案化支撑双层膜。最后，讨论了允许进行改进的荧光活细胞成像的 T-APC 对阵列。
