Bortolon José Ricardo, Silva Junior Antonio José de Almeida, Murata Gilson Masahiro, Newsholme Philip, Curi Rui, Pithon-Curi Tania Cristina, Hatanaka Elaine
Institute of Physical Activity and Sport Sciences, Cruzeiro do Sul University, Rua Galvão Bueno 868, 13° andar, 01506-000 São Paulo, Brazil.
Exp Diabetes Res. 2012;2012:213986. doi: 10.1155/2012/213986. Epub 2012 Aug 13.
In this study we evaluated the onset and resolution of inflammation in control and streptozotocin-induced diabetic rats subjected to a single session of intense exercise. The following measurements were carried out prior to, immediately after, and 2 and 24 hours after exercise: plasma levels of proinflammatory cytokines (TNF-α, IL-1β, IL-6, CINC-2α/β, MIP-3α, and IL-6), immunoglobulins (IgA and IgM), acute phase proteins (CRP and C3), and creatine kinase (CK) activity. We also examined the occurrence of macrophage death by measurements of macrophages necrosis (loss of membrane integrity) and DNA fragmentation. An increase was observed in the concentration of IL-1β (3.3-fold) and TNF-α (2.0-fold) and in the proportion of necrotic macrophages (4.5-fold) in diabetic rats 24 hours after exercise, while the control group showed basal measurements. Twenty-four hours after the exercise, serum CK activity was elevated in diabetic rats but not in control animals. We concluded that lesion and inflammations resulting from intense exercise were greater and lasted longer in diabetic animals than in nondiabetic control rats.
在本研究中，我们评估了单次剧烈运动的对照大鼠和链脲佐菌素诱导的糖尿病大鼠炎症的发生和消退情况。在运动前、运动后即刻、运动后2小时和24小时进行了以下测量：促炎细胞因子（TNF-α、IL-1β、IL-6、CINC-2α/β、MIP-3α和IL-6）、免疫球蛋白（IgA和IgM）、急性期蛋白（CRP和C3）的血浆水平以及肌酸激酶（CK）活性。我们还通过测量巨噬细胞坏死（膜完整性丧失）和DNA片段化来检查巨噬细胞死亡的发生情况。运动后24小时，糖尿病大鼠的IL-1β浓度（3.3倍）和TNF-α浓度（2.0倍）以及坏死巨噬细胞比例（4.5倍）均升高，而对照组显示为基础测量值。运动后24小时，糖尿病大鼠的血清CK活性升高，而对照动物未升高。我们得出结论，与非糖尿病对照大鼠相比，剧烈运动导致的损伤和炎症在糖尿病动物中更严重且持续时间更长。
