Programa de Pós-Graduação em Neurociências, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Brazil.
Brain Res. 2011 Mar 25;1382:173-80. doi: 10.1016/j.brainres.2011.01.063. Epub 2011 Jan 26.
The aim of this study was to evaluate the effects of treadmill training on motor skills and immunoreactivity to tyrosine hydroxylase in the substantia nigra pars compacta and ventral tegmental area from diabetic rats induced by streptozotocin. Male Wistar rats were divided into three groups: control, diabetic and trained diabetic. Treadmill training was performed for 8weeks. Blood glucose concentrations and body weight were evaluated 48h after diabetes induction and every 30days thereafter. Motor skills were evaluated on the rotarod and open field tests. Then, animals were transcardially perfused and the brains were post-fixed, cryoprotected and sectioned in a cryostat. Immunohistochemistry for tyrosine hydroxylase analyses was done in the ventral tegmental area and in the substantia nigra. Motor skills showed that diabetic animals had a decrease in the latency to fall and enhanced number of falls in the rotarod test compared to control and trained diabetic animals. In the open field, diabetic animals had a decrease in the number of crossed squares, rearings and spent a less time moving compared to control and trained diabetic animals. In diabetic animals, optical densitometry of immunohistochemistry showed that tyrosine hydroxylase reaction decreased in the ventral tegmental area and in the neurons and process in the substantia nigra. In the later region, that decrease was reversed by treadmill training. In conclusion, we demonstrated that treadmill training can reverse the loss of the motor skills, which was correlated to tyrosine hydroxylase immunoreactivity in the substantia nigra of diabetic animals without pharmacological treatment.
本研究旨在评估跑步机训练对链脲佐菌素诱导的糖尿病大鼠运动技能和酪氨酸羟化酶免疫反应的影响。雄性 Wistar 大鼠分为三组：对照组、糖尿病组和训练糖尿病组。跑步机训练持续 8 周。在糖尿病诱导后 48 小时和此后每 30 天评估血糖浓度和体重。运动技能通过转棒和旷场测试进行评估。然后，动物进行心脏灌流，大脑用后固定液固定、冷冻保护并在冷冻切片机中切片。酪氨酸羟化酶分析的免疫组织化学在腹侧被盖区和黑质中进行。运动技能显示，与对照组和训练糖尿病组相比，糖尿病动物在转棒测试中摔倒潜伏期缩短，摔倒次数增加。在旷场中，与对照组和训练糖尿病组相比，糖尿病动物穿越方格的次数减少，后肢站立的次数减少，移动的时间减少。在糖尿病动物中，免疫组织化学的光密度测定显示，酪氨酸羟化酶反应在腹侧被盖区和黑质中的神经元和突起中减少。在后一区域，跑步机训练可逆转这种减少。总之，我们证明了跑步机训练可以逆转运动技能的丧失，这与未经药物治疗的糖尿病动物黑质中酪氨酸羟化酶免疫反应有关。
