Department of Respiratory Medicine, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, PR China.
Microb Drug Resist. 2012 Dec;18(6):574-7. doi: 10.1089/mdr.2012.0076. Epub 2012 Aug 28.
The aim of this study was to investigate the in vitro activity of minocycline combined with cefoperazone-sulbactam against carbapenem-resistant Acinetobacter baumannii (CRAB). A total of 53 nonduplicate CRAB were collected from inpatients of intensive care units in three hospitals in China from February 2009 to August 2011. Minimum inhibitory concentrations were determined by the broth microdilution method. The checkerboard method was used to determine whether combinations of the two agents act synergistically. Among the 53 A. baumannii, 64.2% of the isolates were susceptible to amikacin and 69.8% were susceptible to cefoperazone-sulbactam. More than 80% of isolates were resistant to the other agents and no isolates were found resistant to polymyxin B and minocycline. The combination of minocycline and cefoperazone-sulbactam demonstrated synergism in 39 isolates, partial synergism in 11 isolates, and indifference in 3 isolates. No antagonistic interactions were observed. Our study demonstrated that minocycline has good activity against CRAB and the combination of minocycline with cefoperazone-sulbactam had significant synergistic activity against these strains in vitro. The combination of minocycline and cefoperazone-sulbactam may be an alternative option for the treatment of infections caused by CRAB.
本研究旨在探讨米诺环素联合头孢哌酮-舒巴坦对耐碳青霉烯类鲍曼不动杆菌（CRAB）的体外活性。2009 年 2 月至 2011 年 8 月，从中国 3 家医院的重症监护病房住院患者中收集了 53 株非重复的 CRAB。采用肉汤微量稀释法测定最低抑菌浓度。采用棋盘法确定两种药物联合使用是否协同作用。在 53 株鲍曼不动杆菌中，64.2%的分离株对阿米卡星敏感，69.8%的分离株对头孢哌酮-舒巴坦敏感。超过 80%的分离株对其他药物耐药，没有分离株对多黏菌素 B 和米诺环素耐药。米诺环素与头孢哌酮-舒巴坦联合使用在 39 株分离株中表现出协同作用，在 11 株分离株中表现出部分协同作用，在 3 株分离株中表现出无关作用。未观察到拮抗作用。本研究表明，米诺环素对 CRAB 具有良好的活性，米诺环素与头孢哌酮-舒巴坦联合使用对这些菌株具有显著的协同体外活性。米诺环素联合头孢哌酮-舒巴坦可能是治疗 CRAB 感染的一种替代选择。
