Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
Res Vet Sci. 2013 Feb;94(1):73-6. doi: 10.1016/j.rvsc.2012.07.021. Epub 2012 Aug 25.
The ability of different adjuvants to enhance immune responses to intradermal (ID) immunisation with a model antigen was studied in pigs. Immune responses were evaluated with respect to the intensity of systemic and mucosal antibody formation, their isotype characterisation and rate of cell-mediated immunity. These findings were compared with the intensity of adverse local reactions. Six groups of piglets were immunised with keyhole limpet haemocyanin (KLH) antigen alone or in combination with aluminium hydroxide or selected oil-based adjuvants (complete and incomplete Freund's adjuvants, Montanide ISA 206 and Emulsigen). Systemic specific antibody responses were significantly increased following ID administration of antigen together with any of the adjuvants used. IgG antibody responses were most pronounced after the first administration of antigen, being stimulated with both Freund's and Montanide ISA 206 adjuvants. The oil adjuvants also enhanced the cell-mediated immune responses and the levels of local IgA antibodies in the respiratory mucosa. On the other hand, they elicited more pronounced adverse local reactions.
研究了不同佐剂增强模型抗原皮内（ID）免疫的能力在猪。免疫反应的强度进行了评估，包括系统和粘膜抗体的形成、其同种型特征和细胞介导免疫的速度。这些发现与局部不良反应的强度进行了比较。六组小猪单独用钥孔血蓝蛋白（KLH）抗原或与氢氧化铝或选定的油基佐剂（完全和不完全弗氏佐剂、Montanide ISA 206 和 Emulsigen）联合免疫。在 ID 给予抗原的同时使用任何一种佐剂都能显著增强系统特异性抗体反应。IgG 抗体反应在首次给予抗原后最为明显，两种弗氏佐剂和 Montanide ISA 206 佐剂均有刺激作用。油佐剂还增强了细胞介导的免疫反应和呼吸道粘膜局部 IgA 抗体的水平。另一方面，它们引起了更明显的局部不良反应。
