用于心血管手术中制造血管的无细胞胶原蛋白基支架。
Cell-free collagen-based scaffolds used for making blood vessels in cardiovascular surgery.
作者信息
Akhmedov Sh D, Afanas'ev S A, Egorova M V, Andreev S L, Ivanov A V, Rogovskaia Yu V, Usov V Yu, Shvedov A N, Steinhoff G
机构信息
Department of Cardiovascular Surgery Research Institute of Cardiology under the Siberian Branch of the Russian Academy of Medical Sciences.
出版信息
Angiol Sosud Khir. 2012;18(2):7-12.
The present article deals with the technology of obtaining decellularized cell-free collagen-based scaffolds from arterial vessels and surgical assessment of the possibility of experimentally implanting them into the blood system of laboratory animals for experimental purposes. The study was performed on arterial vessels (n=60) and fragments of the human internal thoracic artery (n=20). Described herein is a method of obtaining a connective-tissue matrix of a blood vessel by means of vessel's perfusion for 2-3 hours with detergent solutions. Cell-free collagen-based conduits were implanted to a total of ten dogs. After the operation, the blood flow remained functional. The anastomoses established turned out to be leak-proof and the acellular vessels were able to withstand the haemodynamic load of the arterial blood flow.
本文论述了从动脉血管获取去细胞无细胞胶原蛋白基支架的技术，以及对出于实验目的将其实验性植入实验动物血液系统可能性的手术评估。该研究在动脉血管（n = 60）和人胸廓内动脉片段（n = 20）上进行。本文描述了一种通过用洗涤剂溶液对血管灌注2 - 3小时来获取血管结缔组织基质的方法。将无细胞胶原蛋白基导管总共植入了十只狗体内。手术后，血流保持正常。所建立的吻合口结果是防漏的，并且无细胞血管能够承受动脉血流的血流动力学负荷。
Zotero 插件