School of Chemistry, University of Bristol, Cantock's Close, Bristol, BS8 1TS, UK.
Nat Prod Rep. 2012 Oct;29(10):1111-37. doi: 10.1039/c2np20062g. Epub 2012 Aug 24.
Common to all FASs, PKSs and NRPSs is a remarkable component, the acyl or peptidyl carrier protein (A/PCP). These take the form of small individual proteins in type II systems or discrete folded domains in the multi-domain type I systems and are characterized by a fold consisting of three major α-helices and between 60-100 amino acids. This protein is central to these biosynthetic systems and it must bind and transport a wide variety of functionalized ligands as well as mediate numerous protein-protein interactions, all of which contribute to efficient enzyme turnover. This review covers the structural and biochemical characterization of carrier proteins, as well as assessing their interactions with different ligands, and other synthase components. Finally, their role as an emerging tool in biotechnology is discussed.
所有 FAS、PKS 和 NRPS 的共同点是一个显著的组件，酰基或肽基载体蛋白（A/PCP）。这些在 II 型系统中以单个小蛋白的形式存在，或者在多域 I 型系统中以离散折叠结构域的形式存在，其特征是由三个主要的α-螺旋和 60-100 个氨基酸组成的折叠结构。这种蛋白质是这些生物合成系统的核心，它必须结合和运输各种功能化配体，以及介导众多蛋白质-蛋白质相互作用，所有这些都有助于有效的酶周转。这篇综述涵盖了载体蛋白的结构和生化特性，以及评估它们与不同配体和其他合成酶组件的相互作用。最后，讨论了它们作为生物技术中新兴工具的作用。
