Zini I, Zoli M, Grimaldi R, Pich E M, Biagini G, Fuxe K, Agnati L F
Institute of Human Physiology, University of Modena, Italy.
Neurosci Lett. 1990 Nov 27;120(1):13-6. doi: 10.1016/0304-3940(90)90156-4.
The effect of alpha-difluoromethylornithine (alpha-DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC) the rate limiting enzyme of polyamine biosynthesis, was studied on the astroglial reaction in a model of mechanical brain injury. alpha-DFMO markedly decreased the astroglial activation induced by the microdialysis probe implantation in the striatum of the male rat, as studied by glial fibrillary acidic protein (GFAP) immunocytochemistry. This response was restored by putrescine (20 nmol/ml) administered via the microdialysis probe. These results suggest that the astroglial reaction and the polyamine biosynthesis activation induced by a localized mechanical lesion are causally linked phenomena.
α-二氟甲基鸟氨酸（α-DFMO）是多胺生物合成限速酶鸟氨酸脱羧酶（ODC）的不可逆抑制剂，本研究在机械性脑损伤模型中探讨了其对星形胶质细胞反应的影响。通过胶质纤维酸性蛋白（GFAP）免疫细胞化学研究发现，α-DFMO显著降低了雄性大鼠纹状体内微透析探针植入所诱导的星形胶质细胞活化。通过微透析探针给予腐胺（20 nmol/ml）可恢复这种反应。这些结果表明，局部机械性损伤诱导的星形胶质细胞反应和多胺生物合成激活是因果相关的现象。
