Herdegen T, Walker T, Leah J D, Bravo R, Zimmermann M
II. Physiologisches Institut der Universität Heidelberg, F.R.G.
Neurosci Lett. 1990 Nov 27;120(1):21-4. doi: 10.1016/0304-3940(90)90158-6.
The expression of the protein product encoded by the Krox-24 gene was investigated immunocytochemically in the central nervous system of adult rats. Immunoreactivity (IR) of the KROX-24 protein which is a nuclear transacting transcription factor, showed a pattern of nuclear staining. Basal KROX-24-IR was visible in the superficial layers of spinal dorsal horn and trigeminal nucleus, and in many areas of the brain including the cerebellum, nucleus raphe magnus, colliculi, periaqueductal gray, hypothalamus, geniculate nuclei, caudate putamen, amygdala, hippocampus, lateral septal nucleus, olfactory tubercle and cerebral cortex. Electrical stimulation of sciatic nerve A- and C-fibers, but not of A alpha- and A beta-fibers alone, induced transient expression of KROX-24 in numerous spinal neurons in the termination area of the stimulated nerve. One hour following the onset of this stimulation of the sciatic nerve the distribution of KROX-24-IR was investigated in the brain and compared to untreated rats. In stimulated animals, the KROX-24-IR was induced in many areas including the lateral reticular nucleus, parabrachial nucleus, periaqueductal gray, hypothalamus, amygdala and lateral habenular nucleus.
采用免疫细胞化学方法，对成年大鼠中枢神经系统中Krox - 24基因编码的蛋白质产物表达进行了研究。作为一种核转录调节因子的KROX - 24蛋白的免疫反应性（IR）呈核染色模式。基础KROX - 24 - IR可见于脊髓背角浅层和三叉神经核，以及包括小脑、中缝大核、丘、导水管周围灰质、下丘脑、膝状核、尾壳核、杏仁核、海马、外侧隔核、嗅结节和大脑皮层在内的许多脑区。电刺激坐骨神经的A纤维和C纤维，但单独刺激Aα纤维和Aβ纤维则不会，可诱导受刺激神经终末区域内众多脊髓神经元短暂表达KROX - 24。坐骨神经刺激开始1小时后，研究了KROX - 24 - IR在脑中的分布，并与未处理大鼠进行比较。在受刺激动物中，KROX - 24 - IR在许多区域被诱导表达，包括外侧网状核、臂旁核、导水管周围灰质、下丘脑、杏仁核和外侧缰核。
