电刺激和河豚毒素麻痹对大鼠骨骼肌乙酰胆碱受体抗原特性的影响。

Effects of electrical stimulation and tetrodotoxin paralysis on antigenic properties of acetylcholine receptors in rat skeletal muscle.

作者信息

O'Malley J P, Mills R G, Bray J J

机构信息

Department of Physiology, University of Otago Medical School, Dunedin, New Zealand.

出版信息

Neurosci Lett. 1990 Dec 11;120(2):224-6. doi: 10.1016/0304-3940(90)90044-a.

DOI:10.1016/0304-3940(90)90044-a

PMID:2293108

Abstract

To examine the role of muscle activity in the expression of fetal- and adult-type acetylcholine receptors (AChRs), we studied the effects of muscle stimulation in cell culture and of tetrodotoxin (TTX)-induced paralysis and denervation in adult rat muscles. The AChR content of these muscles was determined using [125I]alpha-bungarotoxin and the proportion of fetal-type receptors was estimated using a radioimmunoprecipitation assay with a myasthenic serum that was highly specific for fetal-type receptors. We found that both stimulated, aneural muscle cells in vitro and inactive muscles in vivo produced predominantly fetal-type AChRs. However the TTX-paralysed muscles had a lower proportion of fetal-type receptors than the denervated muscles. We conclude that neither muscle activity nor innervation alone, but a combination of both, is required for full regulation of AChR antigenicity.

摘要

为了研究肌肉活动在胎儿型和成体型乙酰胆碱受体（AChRs）表达中的作用，我们研究了细胞培养中肌肉刺激以及成年大鼠肌肉中河豚毒素（TTX）诱导的麻痹和去神经支配的影响。使用[125I]α-银环蛇毒素测定这些肌肉的AChR含量，并使用对胎儿型受体具有高度特异性的重症肌无力血清通过放射免疫沉淀试验估计胎儿型受体的比例。我们发现，体外受刺激的无神经肌肉细胞和体内无活性的肌肉主要产生胎儿型AChRs。然而，TTX麻痹的肌肉中胎儿型受体的比例低于去神经支配的肌肉。我们得出结论，对于AChR抗原性的完全调节，肌肉活动和神经支配单独一项都不需要，而是两者的结合才需要。