Department of Bacteriology, Animal Health and Veterinary Laboratories Agency (AHVLA), Addlestone, Surrey, UK.
Zoonoses Public Health. 2013 Sep;60(6):412-25. doi: 10.1111/zph.12009. Epub 2012 Aug 30.
The objective of the study was to evaluate the performance of different combinations of sample type, transport medium and culture methods for the recovery of Campylobacter jejuni and C. coli from broiler flocks at primary production. Boot swabs moistened with one of four different transport media [maximum recovery diluent (n=120), Exeter broth (EX) (n=120), buffered peptone water (n=120) and modified semi-solid Cary-Blair (n=120)], caecal samples (n=40) and faecal samples (n=120) from 40 broiler flocks were compared and sensitivity estimates obtained using a Bayesian model. Samples were cultured onto mCCDA before and after enrichment in EX and incubated microaerobically at 41.5°C. Campylobacter suspect colonies were identified to the species level by multiplex PCR. Results from the Bayesian model indicated that boot swabs after enrichment had higher sensitivity (90-94%) than caecal contents before or after enrichment (84% and 89%, respectively) and faecal samples after enrichment (82%) for the detection of Campylobacter spp., although these differences were not statistically significant. Enrichment significantly increased the sensitivity of boot swab and caecal samples for detection of Campylobacter spp. and C. jejuni, respectively. However, the enrichment of caecal samples resulted in a significant decrease in the sensitivity of these samples for detection of C. coli. There was much greater variation in the sensitivity estimates of the methods for detecting C. coli than for C. jejuni, and the ranking of methods was different between the two species. Boot swabs gave the best sensitivity values for detection of C. jejuni, and enrichment culture of faecal samples was the most sensitive method for detection of C. coli.
本研究的目的是评估不同样本类型、运输培养基和培养方法组合对从肉鸡生产第一线鸡群中回收空肠弯曲菌和大肠弯曲菌的效果。用四种不同的运输培养基（最大回收稀释液（n=120）、埃克塞特肉汤（EX）（n=120）、缓冲蛋白胨水（n=120）和改良半固体卡里-布莱尔（n=120））润湿靴拭子、盲肠样本（n=40）和粪便样本（n=120）来自 40 个肉鸡群进行了比较，并使用贝叶斯模型获得了敏感性估计。在 EX 中富集前后，将样品接种到 mCCDA 上，并在 41.5°C 微需氧条件下孵育。通过多重 PCR 将疑似弯曲菌的可疑菌落鉴定到种水平。贝叶斯模型的结果表明，富集后的靴拭子比盲肠内容物（分别为 84%和 89%）和富集后的粪便样本（82%）对空肠弯曲菌属的检测具有更高的敏感性（90-94%），尽管这些差异没有统计学意义。富集显著提高了靴拭子和盲肠样本检测空肠弯曲菌属的敏感性。然而，盲肠样本的富集导致这些样本检测大肠弯曲菌的敏感性显著降低。检测大肠弯曲菌的方法敏感性估计值的变化比检测空肠弯曲菌属的方法大得多，而且两种方法之间的方法排序也不同。靴拭子检测空肠弯曲菌属的敏感性最高，而粪便样本的富集培养是检测大肠弯曲菌属的最敏感方法。
