[一种研究核定位信号介导的核转位的新方法]
[A novel method for studying nuclear localization signal-mediated nuclear translocation].
作者信息
Deng Peng, Gong Xiaowei, Jiang Yong
机构信息
Department of Pathophysiology, Southern Medical University, Guangzhou, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Aug;32(8):1148-50.
OBJECTIVE
To establish a new method for studying the mechanism of nuclear localization signal (NLS)-mediated nuclear translocation in living cells.
METHODS
The cells were treated with 67 mg/L 3-[(3-Cholamidopropyl)dimethylammonio]propanesulfonate (CHAPS), followed by incubation with 1 g/L wheat germ agglutinin (WGA), and their effects on interferon- γ (IFN-γ)-induced nuclear translocation of signal transducer and activator of transcription 1 (STAT1) were observed.
RESULTS
Treatment with CHAPS alone had no effect on IFN-γ-induced nuclear translocation of STAT1, while this process was blocked by further WGA incubation.
CONCLUSION
We established a new, simple but effective method for studying the mechanism of NLS-mediated nuclear translocation in living cells by perforating the cell membrane with CHAPS treatment.
目的
建立一种研究核定位信号（NLS）介导的活细胞内核转位机制的新方法。
方法
用67mg/L的3-[(3-胆酰胺丙基)二甲基铵]丙烷磺酸盐（CHAPS）处理细胞，随后用1g/L的麦胚凝集素（WGA）孵育，观察其对干扰素-γ（IFN-γ）诱导的信号转导和转录激活因子1（STAT1）核转位的影响。
结果
单独用CHAPS处理对IFN-γ诱导的STAT1核转位没有影响，而进一步用WGA孵育则会阻断这一过程。
结论
我们通过CHAPS处理使细胞膜穿孔，建立了一种新的、简单但有效的研究活细胞中NLS介导的核转位机制的方法。
Zotero 插件