Yuan Ying-Ying, Zeng Zhi-Yong, Chen Jun-Min
The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012 Aug;20(4):922-5.
The aim of this study was to explore the effect of DAPT (N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycinet-butyl ester) on proliferation in vitro of human multiple myeloma cell line RPMI8226 and its underlying mechanism. The proliferation of RPMI8226 cells was detected by CCK-8 method; flow cytometry was employed to assay the cell apoptosis rate;the expressions of Notch1 and Hes1 proteins were detected by Western blot. The results indicated that the proliferation of human RPMI8226 cells significantly decreased after treatment with DAPT 0.5 - 5.0 µmol/L for 24 - 72 h (P < 0.05) in a concentration- and time-dependent manner. DAPT significantly induced apoptosis of RPMI8226 cells (P < 0.05). The expressions of Notch1 and Hes1 proteins were gradually downregulated with the increase of DAPT concentration. It is concluded that the DAPT can inhibit the proliferation of RPMI8226 cells, which may be related with the down-regulation of the protein expression of Notchl and Hes1.
本研究旨在探讨二肽基肽酶抑制剂（N-[N-(3,5-二氟苯乙酰基)-L-丙氨酰基]-S-苯甘氨酸叔丁酯）对人多发性骨髓瘤细胞系RPMI8226体外增殖的影响及其潜在机制。采用CCK-8法检测RPMI8226细胞的增殖情况；运用流式细胞术检测细胞凋亡率；通过蛋白质印迹法检测Notch1和Hes1蛋白的表达。结果表明，用0.5 - 5.0 µmol/L的二肽基肽酶抑制剂处理人RPMI8226细胞24 - 72小时后，细胞增殖显著降低（P < 0.05），呈浓度和时间依赖性。二肽基肽酶抑制剂显著诱导RPMI8226细胞凋亡（P < 0.05）。随着二肽基肽酶抑制剂浓度的增加，Notch1和Hes1蛋白的表达逐渐下调。结论是，二肽基肽酶抑制剂可抑制RPMI8226细胞的增殖，这可能与Notch1和Hes1蛋白表达的下调有关。
