Du Rong-hui, Cao Tan-ze, Lian Zhan, Jin Wu, Qiu Ming
Department of Internal Medicine, Wuhan Institute of Tuberculosis Prevention and Control, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2012 Jul;35(7):507-10.
The aim of this study was to investigate the distributions and relevance of Th1 and Th17 cells (IL-17-producing CD(+)(4) T cell), and the differentiation of Th17 cells in tuberculous pleural effusion.
The percentages of both Th1 and Th17 cells in tuberculous pleural effusion and peripheral blood from 30 patients [male/female 12/18, age 16 - 63 years (average 41.2 year)] with tuberculous pleurisy were determined by flow cytometry, and comparison was made using Student's t test. The regulations of different combinations of IFN-γ, IL-1β, IL-6 and IL-12 on differentiation of Th17 cells were explored. Comparisons of the data between different groups were performed using Kruskal-Wallis one-way analysis of variance on ranks.
Both Th1 [(39 ± 11)% vs (8 ± 3)%; t = 17.37, P < 0.05] and Th17 cells [(2.8 ± 0.9)% vs (0.7 ± 0.3)%; t = 14.78, P < 0.05] were significantly increased in tuberculous pleural effusion compared with peripheral blood. The proportions of Th17 cells were correlated positively with those of Th1 cells both in tuberculous pleural effusion and in peripheral blood (r = 0.61, 0.49, respectively; both P < 0.05). IL-1β or IL-6 promoted the differentiation of Th17 cells, and their combination resulted in further increase of the differentiation of Th17 cells, while IFN-γ and IL-12 reduced the percentages of Th17 cells. Moreover, these two cytokines significantly impaired the promotive effect induced by IL-1β plus IL-6.
This study showed that Th1/Th17 balance existed in tuberculous pleural effusion, and was mainly due to the generation and differentiation of Th17 cells induced by IL-1β and IL-6, but reversed by IFN-γ and IL-12 in tuberculous pleural effusion.
本研究旨在探讨结核性胸腔积液中Th1和Th17细胞（产生白细胞介素-17的CD4+T细胞）的分布及相关性，以及Th17细胞的分化情况。
采用流式细胞术检测30例结核性胸膜炎患者[男/女12/18，年龄16 - 63岁（平均41.2岁）]的结核性胸腔积液及外周血中Th1和Th17细胞的百分比，采用Student's t检验进行比较。探讨干扰素-γ、白细胞介素-1β、白细胞介素-6和白细胞介素-12不同组合对Th17细胞分化的调控作用。不同组间数据比较采用Kruskal-Wallis秩和检验。
与外周血相比，结核性胸腔积液中Th1细胞[(39 ± 11)% vs (8 ± 3)%; t = 17.37, P < 0.05]和Th17细胞[(2.8 ± 0.9)% vs (0.7 ± 0.3)%; t = 14.78, P < 0.05]均显著增加。结核性胸腔积液和外周血中Th17细胞比例均与Th1细胞比例呈正相关（分别为r = 0.61、0.49；均P < 0.05）。白细胞介素-1β或白细胞介素-6促进Th17细胞分化，二者联合可使Th17细胞分化进一步增加，而干扰素-γ和白细胞介素-12降低Th17细胞百分比。此外，这两种细胞因子显著削弱白细胞介素-1β加白细胞介素-6诱导的促进作用。
本研究表明结核性胸腔积液中存在Th1/Th17平衡，主要是由于白细胞介素-1β和白细胞介素-6诱导Th17细胞的产生和分化，但在结核性胸腔积液中被干扰素-γ和白细胞介素-12逆转。
