Zhang Yu, Shi Fang, Li Shi-min, Jin Yun-jie, Wu Hai-tao
Department of Otorhinolaryngology Head and Neck Surgery, Eye Ear Nose Throat Hospital of Fudan University, Shanghai 200031, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2012 Jun;47(6):503-6.
To investigate the sonographic features of the cervical lymph nodes in rabbit VX2 tongue carcinoma model and hyperplasia cervical lymph nodes model using gray scale contrast-enhanced ultrasonography (CEUS) after intravenous administration of SonoVue (a sonographic contrast agent) and to evaluate the potential utility of CEUS in detecting early metastatic cervical lymph nodes in the rabbit VX2 tongue carcinoma model.
Twenty New Zealand rabbits were placed into 2 groups, 12 for node metastasis and 18 for node hyperplasia. Cervical CEUS was performed in the rabbits of both groups before and after intravenous administration of SonoVue(0.6 ml). The phase, pattern, beginning area and echogenicity of enhancement of the lymph nodes on the right neck were observed and recorded. Dissection of the lymph nodes were guided by the marks made during CEUS and examined histopathologically.
On the right side of each rabbit only one lymph node were detected in both groups. All 8 cases in the hyperplasia group showed homogeneous enhancement starting from central, while in the metastasis group one case showed homogeneous enhancement starting from the central, 3 cases showed inhomogeneous enhancement starting from periphery, and 8 cases showed inhomogeneous enhancement starting both from central and periphery. One lymph node was dissected on the right side of the neck in each rabbit in both groups. Pathological examination showed all 12 lymph nodes in the metastatic group were metastatic lymph nodes, and all 8 lymph nodes in the hyperplasia group were hyperplastic lymph nodes.
After intravenous CEUS, metastatic lymph nodes show inhomogeneous enhancement starting from periphery/central or periphery area, while hyperplastic lymph nodes show homogeneous enhancement starting from central area. Metastatic lymph nodes can be characterized as being neoplastic or benign on the basis of the enhancement patterns evaluated by CEUS.
通过静脉注射声诺维（一种超声造影剂）后采用灰阶超声造影（CEUS）研究兔VX2舌癌模型及颈部淋巴结增生模型中颈部淋巴结的超声特征，并评估CEUS在检测兔VX2舌癌模型早期颈部转移淋巴结方面的潜在应用价值。
将20只新西兰兔分为2组，12只用于淋巴结转移模型，18只用于淋巴结增生模型。两组兔在静脉注射声诺维（0.6 ml）前后均行颈部CEUS检查。观察并记录右侧颈部淋巴结增强的时相、方式、起始部位及回声强度。根据CEUS检查时所做标记指导对淋巴结进行解剖，并进行组织病理学检查。
两组中每只兔右侧均仅检测到1个淋巴结。增生组8例均表现为从中央开始的均匀增强，而转移组1例表现为从中央开始的均匀增强，3例表现为从周边开始的不均匀增强，8例表现为从中央和周边开始的不均匀增强。两组中每只兔均在右侧颈部解剖1个淋巴结。病理检查显示转移组12个淋巴结均为转移淋巴结，增生组8个淋巴结均为增生性淋巴结。
静脉注射CEUS后，转移淋巴结表现为从周边/中央或周边区域开始的不均匀增强，而增生性淋巴结表现为从中央区域开始的均匀增强。根据CEUS评估的增强模式，转移淋巴结可被鉴别为肿瘤性或良性。
