编码兔精子膜蛋白的cDNA（RSD-1）的核酸序列及所编码蛋白的结构特性。
Nucleic acid sequence of cDNA (RSD-1) encoding a rabbit sperm membrane protein and structural properties of the encoded protein.
作者信息
Yun B, Miao S, Yan Y, Wang L, Koide S S
机构信息
Institute of Basic Medical Sciences, CAMS, Beijing.
出版信息
Proc Chin Acad Med Sci Peking Union Med Coll. 1990;5(4):181-5.
PMID:2293224
Abstract
The nucleotide sequence of a 2.0 kb cDNA encoding a rabbit sperm tail membrane protein was determined using the chain termination method. Analysis was performed using Sequenase and Klenow enzymes on the target sequence in an M13mp18/19 cloning system. The open reading frame consisted of 438 bp encoding 146 amino acids of the protein, with a calculated Mr of 15507. The hydrophobic amino acid content was 34.9%, and pI was in the vicinity of 5.8.
摘要
采用链终止法测定了编码兔精子尾部膜蛋白的2.0 kb cDNA的核苷酸序列。在M13mp18/19克隆系统中，使用测序酶和Klenow酶对靶序列进行分析。开放阅读框由438 bp组成，编码该蛋白的146个氨基酸，计算得出的分子量为15507。疏水氨基酸含量为34.9%，等电点在5.8左右。
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