Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden.
Int J Gynecol Cancer. 2012 Oct;22(8):1398-406. doi: 10.1097/IGC.0b013e3182664b6b.
Persistent infection with high-risk (HR) human papillomavirus (HPV) is a well-recognized cause of cervical cancer, but little is known about the situation in Laos. The aims of the study were to determine the prevalence of HR-HPV among Lao women and to evaluate the use of a filter paper card (FTA Elute Micro Card) for collection of cervical cells in the humid tropical climate.
This is a cross-sectional study including 1922 women from 3 provinces in Laos. During a gynecological examination, cervical cells were collected and applied to the FTA card followed by HPV typing using a real-time polymerase chain reaction (PCR)-based assay.
Overall, 213 of the 1922 women were positive for HR-HPV (11%). The most common type was the group HPV33/52/58 (3%), followed by the single type 16 (2%) and the group 18/45 (1%), respectively. Only 11 cards (0.6%) did not contain a sufficient amount of genomic DNA for polymerase chain reaction-based analysis.
The prevalence of HR-HPV infections in Laos is similar to other Asian countries, and 40% of the women with an HR-HPV infection will be target of the present HPV vaccines. The FTA card is suitable for collection of cervical cells for HR-HPV typing in tropical conditions. This information is important for planning and establishing primary and secondary prevention of cervical cancer in Laos.
高危型（HR）人乳头瘤病毒（HPV）持续性感染是宫颈癌公认的病因，但老挝的情况尚不清楚。本研究旨在确定老挝女性 HR-HPV 的流行率，并评估在潮湿热带气候下使用滤纸卡片（FTA Elute Micro Card）收集宫颈细胞的情况。
这是一项横断面研究，包括老挝 3 个省的 1922 名女性。在妇科检查过程中，收集宫颈细胞并应用于 FTA 卡，然后使用实时聚合酶链反应（PCR）检测 HPV 类型。
1922 名女性中，共有 213 名 HR-HPV 阳性（11%）。最常见的类型是 HPV33/52/58 组（3%），其次是单一型 16（2%）和 18/45 组（1%）。只有 11 张卡片（0.6%）未包含足够量的基因组 DNA 进行基于 PCR 的分析。
老挝 HR-HPV 感染的流行率与其他亚洲国家相似，目前 HPV 疫苗的目标人群中有 40%的女性感染了 HR-HPV。FTA 卡适合在热带条件下收集宫颈细胞进行 HR-HPV 分型。这些信息对于老挝宫颈癌的一级和二级预防规划和建立很重要。
