Gao Yan, Zhou Lei, Jiang Ying, Guo Ze-hong, Lu Hai-bin, Li Shao-bing
Center of Oral Implantology, Guangdong Provincial Stomatological Hospital & The Affiliated Stomatological Hospital of Southern Medical University, Guangzhou 510280, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2012 Jun;47(6):359-63. doi: 10.3760/cma.j.issn.1002-0098.2012.06.009.
To study the effects of ultraviolet (UV) light-treatment on the physicochemical properties and bioactivity of micro-arc oxidation (MAO) titanium surfaces in vitro.
The pure titanium were prepared using MAO. MAO titanium samples were treated with 15 W bactericidal lamp UVC [λ = (250 ± 20) nm] or 15 W mercury lamp [λ = (360 ± 20) nm] for 24 h under ambient conditions. Three sample groups were prepared: MAO, UVA treated after MAO (MAO + UVA), UVC treated after MAO (MAO + UVC). The surface physicochemical properties were evaluated using scanning electron microscopy (SEM), contact angle measuring device, energy dispersive X-ray spectrometer (EDX), and X-ray diffraction (XRD). Bicinchoninic acid (BCA) based colorimetric detection was used to quantify the percentage of albumin adsorption after 2 h, 6 h, and 24 h incubation on the titanium surfaces. The rates of MG-63 cells attached to each group titanium surfaces were calculated by nucleus immunofluorescence using Hoechst 33342 after 1 h, 2 h, and 4 h incubation. SEM was used to observe cell morphology on titanium surfaces in each group.
No obvious differences in surface topography, TiO(2) crystal and elemental composition were detected on titanium surfaces with or without UV treatment. Statistically significant difference in contact angles among MAO + UVC group (65.34 ± 1.16)°, MAO + UVA group (44.64 ± 1.28)°, and MAO group (3.41 ± 0.48)° were found (P < 0.001). The percentage of albumin adsorption reached the plateau after 2 h incubation on MAO + UVC titanium surfaces (48.16 ± 1.24)%, which was higher than those in MAO [(8.22 ± 2.99)%] and MAO + UVA groups [(5.29 ± 2.27)%, P < 0.001]. The rates of cells attached to the surfaces of MAO + UVC titanium was greater than that on MAO surfaces and MAO + UVA surfaces after 1 h [(40.71 ± 4.08)%], 2 h [(53.72 ± 2.38)%], 4 h [(70.32 ± 2.85)%] incubation (P < 0.05). The MAO + UVC surfaces remarkably enhanced the spread of MG-63 cells, however, there was no significant difference between the group of MAO and MAO + UVA.
Pretreatment of micro-arc oxidation titanium with UVC light considerably improved the surface bioactivity to MG-63 cells, which showed an increase in cellular attachment and spread.
研究紫外线（UV）处理对微弧氧化（MAO）钛表面理化性质及生物活性的体外影响。
采用微弧氧化制备纯钛。将MAO钛样品在环境条件下用15 W杀菌灯UVC[λ = (250 ± 20) nm]或15 W汞灯[λ = (360 ± 20) nm]处理24 h。制备三个样品组：MAO组、MAO后UVA处理组（MAO + UVA）、MAO后UVC处理组（MAO + UVC）。使用扫描电子显微镜（SEM）、接触角测量仪、能量色散X射线光谱仪（EDX）和X射线衍射（XRD）评估表面理化性质。采用基于二辛可宁酸（BCA）的比色法检测钛表面孵育2 h、6 h和24 h后白蛋白吸附的百分比。孵育1 h、2 h和4 h后，使用Hoechst 33342通过细胞核免疫荧光法计算MG-63细胞在每组钛表面的附着率。用SEM观察每组钛表面的细胞形态。
经UV处理和未经UV处理的钛表面在表面形貌、TiO₂晶体和元素组成上未检测到明显差异。MAO + UVC组（65.34 ± 1.16）°、MAO + UVA组（44.64 ± 1.28）°和MAO组（3.41 ± 0.48）°的接触角存在统计学显著差异（P < 0.001）。在MAO + UVC钛表面孵育2 h后白蛋白吸附百分比达到平台期（48.16 ± 1.24）%，高于MAO组[（8.22 ± 2.99）%]和MAO + UVA组[（5.29 ± 2.27）%，P < 0.001]。孵育1 h[（40.71 ± 4.08）%]、2 h[（53.72 ± 2.38）%]、4 h[（70.32 ± 2.85）%]后，MAO + UVC钛表面的细胞附着率大于MAO表面和MAO + UVA表面（P < 0.05）。MAO + UVC表面显著增强了MG-63细胞的铺展，然而，MAO组和MAO + UVA组之间没有显著差异。
用UVC光预处理微弧氧化钛可显著提高其对MG-63细胞的表面生物活性，表现为细胞附着和铺展增加。
