实验性自身免疫性葡萄膜炎的啮齿动物模型
Rodent models of experimental autoimmune uveitis.
作者信息
Agarwal Rajeev K, Silver Phyllis B, Caspi Rachel R
机构信息
Laboratory of Immunology, National Eye Institute, NIH, Bethesda, MD, USA.
出版信息
Methods Mol Biol. 2012;900:443-69. doi: 10.1007/978-1-60761-720-4_22.
Abstract
The model of experimental autoimmune uveitis (EAU) in mice and in rats is described. EAU targets immunologically privileged retinal antigens and serves as a model of autoimmune uveitis in humans as well as a model for autoimmunity in a more general sense. EAU is a well-characterized, robust, and reproducible model that is easily followed and quantitated. It is inducible with synthetic peptides derived from retinal autoantigens in commonly available strains of rats and mice. The ability to induce EAU in various gene-manipulated, including HLA-transgenic, mouse strains makes the EAU model suitable for the study of basic mechanisms as well as in clinically relevant interventions.
摘要
本文描述了小鼠和大鼠实验性自身免疫性葡萄膜炎（EAU）模型。EAU以免疫赦免的视网膜抗原为靶点，不仅是人类自身免疫性葡萄膜炎的模型，从更广泛意义上来说也是自身免疫的模型。EAU是一个特征明确、稳定且可重复的模型，易于追踪和量化。在常用的大鼠和小鼠品系中，用源自视网膜自身抗原的合成肽即可诱导出EAU。在包括HLA转基因小鼠品系在内的各种基因操作小鼠品系中诱导EAU的能力，使得EAU模型适用于基础机制研究以及临床相关干预研究。
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