National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, People's Republic of China.
Am J Bot. 2012 Sep;99(9):e357-61. doi: 10.3732/ajb.1200056. Epub 2012 Aug 29.
Microsatellite markers within regulators of heat stress transcription factors were identified in the Populus trichocarpa genome, and then developed for P. simonii to investigate the genetic diversity of germplasm resources and to further identify favorable alleles significantly associated with stress-resistant traits.
Thirty-five novel microsatellite markers were identified from genes controlling heat stress transcription factors in P. simonii using a Sanger sequencing protocol. Polymorphisms in 48 individuals from 16 populations of P. simonii revealed that the number of alleles per locus ranged from two to nine with an average of 4.6; the observed heterozygosity and expected heterozygosity per locus varied from 0.143 to 0.857 and from 0.257 to 0.948, respectively.
The new polymorphic markers developed during this study will facilitate the construction of genetic linkage maps and will aid in marker-assisted breeding of a new germplasm with desirable abiotic stress resistance in Populus species.
在杨树基因组中鉴定出热应激转录因子调控因子内的微卫星标记，然后开发用于毛白杨的微卫星标记，以研究遗传资源的遗传多样性，并进一步鉴定与抗逆性状显著相关的有利等位基因。
利用 Sanger 测序方案，从控制毛白杨热应激转录因子的基因中鉴定出 35 个新的微卫星标记。对来自 16 个毛白杨种群的 48 个个体进行多态性分析表明，每个位点的等位基因数从 2 到 9 不等，平均为 4.6；每个位点的观测杂合度和期望杂合度分别从 0.143 到 0.857 和从 0.257 到 0.948 不等。
本研究开发的新多态性标记将有助于构建遗传连锁图谱，并有助于毛白杨物种中具有理想抗非生物胁迫新种质的标记辅助选择育种。
