Hals E
Department of Cariology and Endodontics, Dental Faculty, University of Bergen, Norway.
Scand J Dent Res. 1990 Oct;98(5):380-6. doi: 10.1111/j.1600-0722.1990.tb00988.x.
Sections, 60-180 microns thick, of permanent teeth, intact or with minor fillings, were cut parallel or transversal to the course of the dentinal tubules. Some teeth were demineralized, embedded in paraffin wax, cut in series, and stained with toluidine blue pH 5.6, hematoxylin-eosin, or PAS. Altogether, sections of 90 teeth were examined by ordinary and polarized light microscopy and contact microradiography. Alternating light and dark bands, usually 8-30 microns wide, extended from the pulpal border along the course of the tubules, gradually increasing in width, fading in the mantle dentin. Cross-sectioned bands appeared as dark and light "discs". This was the background for the term "columns". In the dark columns, usually exhibiting crowding of tubules, the intertubular dentin was dark and radiolucent; in the light columns, light and radiopaque. Dark columns contained a high amount of GAGs, assumed to have exerted an inhibitory effect on their mineralization. The columns probably represent structural elements in the architecture of dentin, possibly offering resistance against mechanical stress. It was suggested that the columns might depict functional differences in the matrix secretion and mineralization capacities of odontoblasts.
切取厚度为60 - 180微米的恒牙切片，这些牙齿完整或带有小的填充物，切片方向与牙本质小管的走向平行或垂直。一些牙齿经过脱矿处理，嵌入石蜡，连续切片，并用pH 5.6的甲苯胺蓝、苏木精 - 伊红或过碘酸雪夫试剂染色。总共90颗牙齿的切片通过普通光镜、偏光显微镜和接触式显微放射摄影进行检查。明暗交替的条纹，通常宽8 - 30微米，从牙髓边缘沿小管延伸，宽度逐渐增加，在罩牙本质中逐渐消失。横切的条纹呈现为明暗相间的“圆盘”。这就是“柱”这一术语的由来。在暗柱中，通常小管密集，管间牙本质颜色深且射线可透过；在亮柱中，颜色浅且射线不透。暗柱含有大量糖胺聚糖，推测对其矿化有抑制作用。这些柱可能代表牙本质结构中的结构元素，可能对机械应力具有抵抗力。有人提出这些柱可能描绘了成牙本质细胞在基质分泌和矿化能力方面的功能差异。
