[胡萝卜软腐果胶杆菌胡萝卜软腐亚种中flgK基因的功能]

[Function of flgK gene in Pectobacterium carotovorum subsp, carotovorum].

作者信息

Yang Zhongling, Deng Yamin, Du Shuo, Li Ting, Jiang Huan, Liu Fengquan, Fan Jiaqin

机构信息

College of Plant Protection, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Wei Sheng Wu Xue Bao. 2012 Jun 4;52(6):703-9.

PMID:22934350

Abstract

OBJECTIVE

To investigate functions of flgKpcc gene in Pectobacterium carotovorum subsp. carotovorum (P. c. c).

METHODS

The gene knock-out mutant deltaflgKpcc and complemented strain deltaflgKpcc-KH were generated by biparental mating and their phenotypes including cell morphology, motility, pathogenic factors, and pathogenicity were investigated.

RESULTS

Non-flagellum, cell precipitation in the culture and significantly attenuated motility on 0.3% semisolid medium were observed in deltaflgKpcc compared to Pectobacterium carotovorum subsp. carotovorum S1. In addition, significant decrease in cellulase and protease activity, biofilm formation, and pathogenicity on host plant were found in deltaflgKpcc. While there were no apparent difference in growth rate in vitro, deltaflgKpcc-KH, the complementation strain, restored the phenotype of deltaflgKpcc to the wild type level.

CONCLUSION

The gen of flgKpcc not only influences the cell motility, but also pathogenic factors to lead to the decreased pathogenicity in Pectobacterium carotovorum subsp. Carotovorum.

摘要

目的

研究胡萝卜软腐果胶杆菌胡萝卜软腐亚种（P. c. c）中flgKpcc基因的功能。

方法

通过双亲接合产生基因敲除突变体deltaflgKpcc和互补菌株deltaflgKpcc-KH，并研究它们的表型，包括细胞形态、运动性、致病因子和致病性。

结果

与胡萝卜软腐果胶杆菌胡萝卜软腐亚种S1相比，deltaflgKpcc中观察到无鞭毛、培养物中细胞沉淀以及在0.3%半固体培养基上运动性显著减弱。此外，deltaflgKpcc中纤维素酶和蛋白酶活性、生物膜形成以及对寄主植物的致病性显著降低。虽然体外生长速率没有明显差异，但互补菌株deltaflgKpcc-KH将deltaflgKpcc的表型恢复到野生型水平。