Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
Department of Horticultural Biotechnology and Institute of Life Science & Resources, Kyung Hee University, Yongin 441-701, Republic of Korea.
Microbiology (Reading). 2013 Jul;159(Pt 7):1487-1496. doi: 10.1099/mic.0.067280-0. Epub 2013 May 15.
Pectobacterium carotovorum subsp. carotovorum is a well-known plant pathogen that causes severe soft rot disease in various crops, resulting in considerable economic loss. To identify pathogenicity-related factors, Chinese cabbage was inoculated with 5314 transposon mutants of P. carotovorum subsp. carotovorum Pcc21 derived using Tn5 transposon mutagenesis. A total of 35 reduced-virulence or avirulent mutants were isolated, and 14 loci were identified. The 14 loci could be functionally grouped into nutrient utilization (pyrD, purH, purD, leuA and serB), production of plant cell-wall-degrading enzymes (PCWDEs) (expI, expR and PCC21_023220), motility (flgA, fliA and flhB), biofilm formation (expI, expR and qseC), susceptibility to antibacterial plant chemicals (tolC) and unknown function (ECA2640). Among the 14 genes identified, qseC, tolC and PCC21_023220 are novel pathogenicity factors of P. carotovorum subsp. carotovorum involved in biofilm formation, phytochemical resistance and PCWDE production, respectively.
胡萝卜软腐果胶杆菌亚种胡萝卜软腐亚种是一种众所周知的植物病原体，可导致各种作物发生严重的软腐病，造成相当大的经济损失。为了鉴定与致病性相关的因子，本研究采用 Tn5 转座子诱变技术，对白菜接种了源自胡萝卜软腐果胶杆菌亚种胡萝卜软腐亚种 Pcc21 的 5314 个转座子突变体。共分离出 35 个减毒或无毒突变体，并鉴定出 14 个基因座。这 14 个基因座可以在功能上分为营养物质利用（pyrD、purH、purD、leuA 和 serB）、植物细胞壁降解酶（PCWDEs）的产生（expI、expR 和 PCC21_023220）、运动性（flgA、fliA 和 flhB）、生物膜形成（expI、expR 和 qseC）、对植物抗菌化学品的敏感性（tolC）和未知功能（ECA2640）。在所鉴定的 14 个基因中，qseC、tolC 和 PCC21_023220 分别是参与生物膜形成、植物化学抗性和 PCWDE 产生的胡萝卜软腐果胶杆菌亚种胡萝卜软腐亚种的新的致病性因子。
