Lefrère J J, de Montalembert M, Mariotti M, Girot R, Salmon C, Rouger P, Rey J
Institut National de Transfusion Sanguine, Paris, France.
Vox Sang. 1990;59(4):218-21. doi: 10.1111/j.1423-0410.1990.tb00240.x.
The risk of infection with human immunodeficiency virus (HIV) by transfusion is not totally eliminated, since contaminated blood given before seroconversion to HIV is not detected on the actual biological screening. We used the polymerase chain reaction (PCR) assay (with one primer pair in the gag region and two in the pol region) to detect HIV DNA sequences in 30 seronegative polytransfused thalassemic patients and in 60 seropositive individuals (used as positive controls). We did not observe PCR-positive HIV-antibody-negative results in seronegative polytransfused patients.
由于在实际生物学筛查中无法检测到在血清转化为HIV之前输入的受污染血液，因此输血感染人类免疫缺陷病毒（HIV）的风险并未完全消除。我们使用聚合酶链反应（PCR）检测法（在gag区域使用一对引物，在pol区域使用两对引物）检测30例多次输血的血清阴性地中海贫血患者和60例血清阳性个体（用作阳性对照）中的HIV DNA序列。在多次输血的血清阴性患者中，我们未观察到PCR阳性但HIV抗体阴性的结果。
