Department of Stomatology, Zhongshan Hospital, Fudan University, Shanghai, China.
J Periodontal Res. 2013 Apr;48(2):203-12. doi: 10.1111/j.1600-0765.2012.01521.x. Epub 2012 Aug 31.
BACKGROUD AND OBJECTIVE: Genetic factors may influence the colonization of pathogenic bacteria, therefore increasing the risk for the initiation and development of periodontal disease. The present study was carried out to investigate the association of CD14-260 polymorphisms, subgingival microbiota, and gingival crevicular fluid (GCF) cytokine levels with cyclosporine A (CsA)-induced gingival overgrowth (GO) in renal transplant patients.
A total of 204 patients were dichotomized into two groups: 124 with GO and 80 without GO. The CD14-260 polymorphisms were measured using an allele-specific PCR method. The levels of periodontal pathogens were determined by real-time PCR of subgingival samples. GCF levels of IL-1β and sCD14 were detected by ELISA.
The frequency of CD14-260 genotype CT + TT was found to be similar in both groups. Patients with GO presented increased prevalence of Pg, Td, and Tf (red complex) and significantly higher levels of interleukin -1β than those without GO. Patients with GO carrying CT + TT genotypes were found to have higher frequencies of Pg, Td, and Tf than those carrying the CC genotype. Furthermore, in the presence of red complex, CT + TT genotypes were associated with higher interleukin -1β levels and severe GO. Multiple logistic regression analysis demonstrated that the severity of GO is not dependent on age, gender and pharmacological variables, being only associated with CD14-260 genotype and red complex periodontopathogens.
No association between CD14-260 polymorphisms and the prevalence of GO was revealed in renal transplant patients administered CsA. However, CD14-260 CT + TT genotypes are associated with the prevalence of red complex periodontopathogens in patients with GO, and may thus play some role in the development of severe CsA-induced GO.
遗传因素可能影响致病菌的定植，从而增加牙周病发病和发展的风险。本研究旨在探讨 CD14-260 多态性、龈下微生物群和龈沟液（GCF）细胞因子水平与环孢素 A（CsA）诱导的肾移植患者牙龈过度生长（GO）的关系。
共将 204 例患者分为两组：124 例有 GO，80 例无 GO。采用等位基因特异性 PCR 法检测 CD14-260 多态性。通过实时 PCR 检测龈下样本中牙周致病菌的水平。通过 ELISA 检测 GCF 中白细胞介素-1β和 sCD14 的水平。
两组 CD14-260 基因型 CT+TT 的频率相似。GO 组患者Pg、Td 和 Tf（红色复合体）的患病率增加，GCF 中白细胞介素-1β水平明显高于无 GO 组。GO 患者携带 CT+TT 基因型的频率高于携带 CC 基因型的患者。此外，在存在红色复合体的情况下，CT+TT 基因型与更高水平的白细胞介素-1β和严重的 GO 相关。多变量逻辑回归分析表明，GO 的严重程度与年龄、性别和药物变量无关，仅与 CD14-260 基因型和红色复合体牙周病原体相关。
在接受 CsA 治疗的肾移植患者中，未发现 CD14-260 多态性与 GO 的患病率之间存在关联。然而，CD14-260 CT+TT 基因型与 GO 患者红色复合体牙周病原体的患病率相关，因此可能在严重 CsA 诱导的 GO 的发展中起一定作用。
